Team:ITESM Mexico/Results

From 2011.igem.org

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After returning from Indianapolis, the team continued to work as intended, however most of the materials had been already exhausted and the viability of the competent cells was questionable after more than 50 recultures.
After returning from Indianapolis, the team continued to work as intended, however most of the materials had been already exhausted and the viability of the competent cells was questionable after more than 50 recultures.
We suspected that after so many recultures the bacteria were no longer competent for transformation; to check this we prepared minipreps in order to review if the pieces were on the supposed cells.
We suspected that after so many recultures the bacteria were no longer competent for transformation; to check this we prepared minipreps in order to review if the pieces were on the supposed cells.

Revision as of 02:49, 29 October 2011

ITESM MÉXICO

SensE.coli

Igem Itesm


Results


Diapositiva1.JPG
Diapositiva2.JPG
Diapositiva 3a.jpg
Diapositiva3.JPG
Diapositiva4.JPG
IGEM results Página 1.jpg
IGEM results Página 2.jpg
IGEM results Página 3.jpg
IGEM results Página 4.jpg

After returning from Indianapolis, the team continued to work as intended, however most of the materials had been already exhausted and the viability of the competent cells was questionable after more than 50 recultures. We suspected that after so many recultures the bacteria were no longer competent for transformation; to check this we prepared minipreps in order to review if the pieces were on the supposed cells.

SAGE DNA image.png

PCR product analysis showing the last status of the parts within the cells


SAM 7501.JPG

Picture of the last cell culture made on 28/Oct/11 Pink colonies are untransformed bacteria/White colonies are transformed bacteria. Although the number of sucessfully transformed colonies is small, they were picked up and grown in another media.


Due to the complications Post successfull ligation of the parts, we will use two procedures to ensure that the parts are present in the cells