"
Team:ITESM Mexico/Parts submitted
From 2011.igem.org
| Line 2: | Line 2: | ||
| - | + | '''Part Registry''' | |
| Line 59: | Line 59: | ||
| - | + | ''' Sequences and description''' | |
| - | + | ||
| - | ''' | + | |
| Line 68: | Line 66: | ||
Sequence | Sequence | ||
| + | |||
'''GAATTCGCGGCCGCTTCTAG'''ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT | '''GAATTCGCGGCCGCTTCTAG'''ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT | ||
GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT | GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT | ||
| Line 87: | Line 86: | ||
Sequence | Sequence | ||
| + | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTTGGATTTGGGTATCA'''TACTAGTAGCGGCCGCTGCAG''' | '''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTTGGATTTGGGTATCA'''TACTAGTAGCGGCCGCTGCAG''' | ||
| Line 96: | Line 96: | ||
Sequence | Sequence | ||
| + | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT | '''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT | ||
'''TACTAGTAGCGGCCGCTGCAG''' | '''TACTAGTAGCGGCCGCTGCAG''' | ||
| Line 106: | Line 107: | ||
Sequence | Sequence | ||
| + | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT | '''GAATTCGCGGCCGCTTCTAGAG'''AGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT | ||
'''TACTAGTAGCGGCCGCTGCAG''' | '''TACTAGTAGCGGCCGCTGCAG''' | ||
| Line 115: | Line 117: | ||
Sequence | Sequence | ||
| + | |||
'''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTATTTGGCATTCCCAA'''TACTAGTAGCGGCCGCTGCAG''' | '''GAATTCGCGGCCGCTTCTAGAG'''AAGCGCAGTAATTCACCTCTATTTGGCATTCCCAA'''TACTAGTAGCGGCCGCTGCAG''' | ||
Revision as of 02:01, 29 September 2011
ITESM MÉXICO
SensE.coli
Part Registry
| Name | Description | Type | Designer | Length | Lenght (prefix;suffix) |
| BBa_K587000 | RecA | Protein coding | David Maycotte/Patricia Campos | 1062 | 1103 |
| BBa_K587001 | Crxst | Regulatory | David Maycotte | 35 | 78 |
| BBa_K587002 | Tast | Regulatory | David Maycotte | 80 | 123 |
| BBa_K587003 | Tawk | Regulatory | David Maycotte | 79 | 122 |
| BBa_K587004 | Crxwk | Regulatory | David Maycotte | 35 | 78 |
| BBa_K587005 | Itast | Regulatory | David Maycotte | 81 | 124 |
Sequences and description
- RecA
- Protein coding sequence
Sequence
GAATTCGCGGCCGCTTCTAGATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT CGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGC AGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCT GTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCAC TGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTG AAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAA TTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAA ATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATC GAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAG ATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTT TTAATACTAGTAGCGGCCGCTGCAG
- Crxst
- This DNA sequence acts as a lock for the low arabinose concentration regulation path.
Sequence
GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTTGGATTTGGGTATCATACTAGTAGCGGCCGCTGCAG
- Tast
- This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.
Sequence
GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT TACTAGTAGCGGCCGCTGCAG
- Tawk
- This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.
Sequence
GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT TACTAGTAGCGGCCGCTGCAG
- Crxwk
- This DNA sequence acts as a lock for the high arabinose concentration regulation path.
Sequence
GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTATTTGGCATTCCCAATACTAGTAGCGGCCGCTGCAG
- Itast
- This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.
Sequence
GAATTCGCGGCCGCTTCTAGAGTCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA TACTAGTAGCGGCCGCTGCAG
