Team:IIT Madras/Notebook/Protocols

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bar iGEM 2011 - Home Page Indian Institute of Technology - Madras

Protocols


Preperation of competent DH5alpha cells
Transformation
Miniprep using alkaline lysis buffers
Agarose Gel Electrohoresis
Restriction digestion
Gel Elusion
PCR
PCR_purification
Ligation