Team:IIT Madras/Data page

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bar iGEM 2011 - Home Page Indian Institute of Technology - Madras


Data for our new parts

    Main PagePcstA_RFP generator, BBa_K572001: Carbon stress induced promoter when induced by Carbon stress produces RFP. RFP production was seen to increase with decrease in substrate concentration.
    Main PageProteorhodopsin, BBa_K572005: Gene expressing proteorhodopsin (Green light absorbing Proteorhodopsin), native to Marine Gamma Proteobacterium. It has light dependent proton pumping activity.
    Main PageLight based Cloning vector, BBa_K572100: Cloning Plasmid based on pSB1C3, Chloramphenicol is replaced by Proteorhodopsin. This plasmid can be used for light based screening.
    Main PageLight based Expression vector, BBa_K572200: designed based on pSB1C3, for improved expression in carbon limiting conditions, using light as substrate

Data for pre-existing parts

    ExperiencePcstA (glucose-repressible promoter), BBa_K118011: This is a carbon stress induced promoter. We characterized this part by designing and expressing a composite part BBa_K572001, which expressed RFP. We found increasing expression for decreasing substrate concentration.

We've also characterized:

    Main PageMouse Beta Carotene Dioxygenase, BBa_K572009: Gene coding for Beta carotene dioxygenase, it converts beta carotene to All-trans retinal which is the chromophore in Proteorhodopsin. We tried to characterize Beta carotene utilization efficiency of this part, for various media conditions.