Team:Hunter-NYC/Project

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== '''Overall project''' ==
== '''Overall project''' ==
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Your abstract
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We have designed a fusion protein consisting of a domain that binds strongly to zinc, cobalt and cadmium and the
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C-terminus of the Pseudomonas sp. lipase (PML), which acts as a secretion tag for E. coli cells containing the lipBCD
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genes from S. marcescens.  We would like our system to be as sustainable and low-cost as possible so that it, or an
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improved version of it, would be practical and applicable in developing nations where contaminated water supplies are
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especially problematic.  We hope that using a protein secretion system will help us achieve that goal by providing us
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+
with an easy, low energy means to purify the metal filtering fusion protein.  In this scheme, the same population of E.
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coli can be used to produce our protein multiple times since the protein would accumulate in the growth media and not
 +
within the cells, in which case we would need to lyse the E. coli in order to prepare each filter.
== Project Details==
== Project Details==

Latest revision as of 00:36, 24 August 2011


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
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Contents

Overall project

We have designed a fusion protein consisting of a domain that binds strongly to zinc, cobalt and cadmium and the C-terminus of the Pseudomonas sp. lipase (PML), which acts as a secretion tag for E. coli cells containing the lipBCD genes from S. marcescens. We would like our system to be as sustainable and low-cost as possible so that it, or an improved version of it, would be practical and applicable in developing nations where contaminated water supplies are especially problematic. We hope that using a protein secretion system will help us achieve that goal by providing us with an easy, low energy means to purify the metal filtering fusion protein. In this scheme, the same population of E. coli can be used to produce our protein multiple times since the protein would accumulate in the growth media and not within the cells, in which case we would need to lyse the E. coli in order to prepare each filter.

Project Details

Part 2

The Experiments

Part 3

Results