Team:HUST-China

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<b>>>&nbsp; Welcome to HUST's Super E.coli Architect project!'</b></font>
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      <font size=5 ><strong>Welcome </strong></font><br>
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      &nbsp;&nbsp;&nbsp;Wuhan, composed of the three towns of Wuchang, Hankou, and Hanyang, is the capital of Hubei Province. Two bridges link the three towns, separated by the Yangzi River and Hanshui River, and because these municipalities are so closely connected by waterways, Wuhan is also called the "city on rivers."<br>
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&nbsp;&nbsp;&nbsp;In addition, being regarded as A Thoroughfare to Nine Provinces, Wuhan is the most significant water hub and rail transportation and communication of China. Today, Wuhan has been the political, economic, and cultural center of Central China.
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    <font size="5"><strong>Project 1(the web project):</strong></font><p>
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      &nbsp;&nbsp;&nbsp;China's wine culture has developed for thousands of years,and the wine as a traditional drink is closely
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linked with people's life. But excessive drinking will bring a lot of inconvenience, causing many social
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problems. For example, in recent years, drunk driving has become a ferocioustraffic killer.Therefore,we hope to
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develop a kind of engineering bacteria, which can reproduct in the human body , and has the ability of
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      <font size=5 ><strong>Introduction to SEA project</strong></font><br>
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decomposition of excess alcohol function.<br>
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    &nbsp;&nbsp;&nbsp;As the host bacteria of exogenous gene expression,lactobacillus' superiority is mainly manifested in the
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following:1.lactobacillus can colonize in the respiratory system, digestive system and urinary system, playing
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an important role in maintain ecological balance; 2.lactobacillus  has confirmed that has no pathogenicity in
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      &nbsp;&nbsp;&nbsp;Super E.coli Architect (SEA) is a kind of design software for engineering bacteria and offers a convenient and efficient design platform, having capability to integrate scattered simple ideas into a complete solution. <br>
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food industry's various fields of long-term application, is recognized as the security level (GRAS, generally
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&nbsp;&nbsp;&nbsp;However, most existing synthetic biology softwares involve merely such specific functions as finding genes, metabolism pathways, and biobrick design, and it is just because of the intense specificity of those softwares that they are limited only to scientific researchers.<br>
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recognized as safe) microorganisms.As a result , we choose the lactic acid bacteria as recipients in gene
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engineering.<br>
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  &nbsp;&nbsp;&nbsp;In the human body 's metabolism process, the alcohol is mainly catalyzed into acetaldehyde acetaldehyde by
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alcohol dehydrogenase ( ADH) in liver, further by acetaldehyde dehydrogenase (ALDH ) catalytic transformation
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into acetic acid.We found two genes expressing of ADH and ALDH, ADH1B2 and ALDH2, hoping to put them into
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BioBrick, introducing into Lactobacillus expression. At the same time we will introduce promoter of lactate
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detection as well as self-cracking mechanism, improving the gene pathway.<p>
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<font size="5"><strong>Project 2(the software project):</strong></font><p>
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&nbsp;&nbsp;&nbsp;Super E.coli Architect is a design software of engineering bacteria. It offers a convenient and efficient  
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design platform,which contributes to making simple ideas into a complete solutions. The existing synthetic biology softwares  
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involve some specific functions such as finding genes and metabolism pathways, modeling and biobrick design.And these softwares
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are often oriented to scientific researchers. But we think that thereis a hope in synthetic biology to turn the science of artificial
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biosystem into a engineering program having a standard process. Therefore, we constructed a software, Super E.coli Architect,
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oriented to designers more than scientists.Providing all the necessary technical support,SuperE.coli Architect can release
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desighners from technical details,helping them work as the architects,allowing them to take more attention into constructing
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kinds engineering bacterias for solving practical problems.<br>
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<table>
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&nbsp;&nbsp;&nbsp; In order to make synthetic biology softwares more available to common designers who would like to do jobs on synthetic biology but might be not so familiar with it as scientists do, we are determined to alter the science of artificial biosystem into an engineering program with the standard process. <br>
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<tr><td colspan=7><a href="CADgene/index.php" target=_blank><Img src="img/CAD_logo.jpg" width="843" height="100" style='border-style:none'></a></td>
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&nbsp;&nbsp;&nbsp;At the thought above, we constructed the software, SEA, for not only scientists but also common designers. <br>  
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<td width="205" rowspan="2"><a href="img/miRNA-TF-1.jpg"  title="mi-RNA-TF" class="MagicZoom"><Img src="img/miRNA-TF.jpg" width="200" height="180" style='border-style:none' align="top"></a><a href="http://www.biomedcentral.com/1752-0509/4/10" target="_blank"><center>>>access</center></a></td>
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&nbsp;&nbsp;&nbsp;Fortunately, it has been proved that the SEA we have developed is able to release designers from technical details by helping them work as specific architects and allowing them to take more attention to constructing engineering bacteria to solve practical problems.
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<a href="http://datf.cbi.pku.edu.cn" target=_blank><Img src="img/datf.jpg" width="200" height="100" style='border-style:none'></a>
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<a href="http://planttfdb.cbi.pku.edu.cn" target=_blank><Img src="img/planttfdb.jpg" width="200" height="100" style='border-style:none'></a>
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<font size=5 face='GungsuhChe'><strong>We Are Here</strong></font><br>
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<font size=3.5 face='GungsuhChe'><strong>Acknowledgment</strong></font><br>
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<a href='http://qiming.hust.edu.cn/'><img  src='https://static.igem.org/mediawiki/2011/4/42/Hust_acknowledgment_01.png' width=200px /></a>
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<a href='http://english.hust.edu.cn/170_overview.html'><img src='https://static.igem.org/mediawiki/2011/1/17/Hust_acknowledgment_02.png' width=200px /> </a>
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<a href='https://2011.igem.org/Team:WHU-China'><img src='https://static.igem.org/mediawiki/2011/0/01/Hust_acknowledgment_04.jpg' width=100px /> </a>
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<a href='https://2011.igem.org/Team:USTC-China'><img src='https://static.igem.org/mediawiki/2011/5/51/Hust_acknowledgment_03.png' width=100px /> </a>
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<a href="img/sbp-1.jpg"  title="sbp" class="MagicZoom"><Img src="img/sbp.jpg" width="200" height="180" style='border-style:none' align="top"></a><a href="http://www.ncbi.nlm.nih.gov/pubmed/18495384" target="_blank"><center>>>access</center></a>
 
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<a href="http://www.ncbi.nlm.nih.gov/pubmed/18663367/" target=_blank><img src="img/DTNBP1.jpg" width="200" height="100" style='border-style:none'></a>
 
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<a href="http://bioinfo.mc.vanderbilt.edu/ERGR/" target=_blank><Img src="img/ERGR.jpg" width="200" height="100" style='border-style:none'></a>
 
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