Team:HKUST-Hong Kong/data.html

From 2011.igem.org

Revision as of 12:11, 5 October 2011 by Lax (Talk | contribs)


Data Page



  • Data For Our Favorite New Parts:
1. Main Page –oriR101 & repA101-ts, BBa_K524000: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C,

  • Parts Constructed But Not Characterized
1. Main Page –pLac + RBS + sfGFP1-10, BBa_K524001: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.

2. Main Page –pLac + RBS + sfGFP11 + double terminator, BBa_K524002: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.

3. Main Page – nadE gene + double terminator, BBa_K524003: an essential gene that encodes constitutive expressed NAD+ synthatase.

4. Main Page –pir gene, BBa_K524004: the pir gene encodes the autogenously regulated pi protein.

5. Main Page – pToolkit, BBa_K524005: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.

6. Main Page –RBS + sfGFP1-10, BBa_K524006: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.

7. Main Page –RBS + sfGFP11 + double terminator, BBa_K524007: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.

8. Main Page – bcr CDS + double terminator B0015, BBa_K524100: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degree of resistance to 5 antibiotics.

Top


Home

Our Project

Overview | Data Page
Experiments and Results
Strain Construction | Culture Tests | Modeling
Miscellaneous
Notebook

iGEM Resources

Acknowledgements
The Team
iGEM Member List | Contributions
Achievements
Medal Requirements | BioSafety
Biobricks
Master List & Characterization Data

Human Practice

Workshop | Survey