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Team:HKUST-Hong Kong
From 2011.igem.org
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<p align=justify style="margin: 20px 20px 20px 20px"> | <p align=justify style="margin: 20px 20px 20px 20px"> | ||
| - | <b> | + | <b>Greetings from the <b>2011 HKUST iGEM Team</b>!</b><br><br> |
Welcome to our Wiki page!<br></p> | Welcome to our Wiki page!<br></p> | ||
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<p align-justified style="margin: 20px 20px 20px 20px"> | <p align-justified style="margin: 20px 20px 20px 20px"> | ||
This is the 4<sup>th</sup> time HKUST has participated in this international synthetic biology competition.<br> | This is the 4<sup>th</sup> time HKUST has participated in this international synthetic biology competition.<br> | ||
| - | Thanks to our instructors' helpful advice, considerate advisors, and great enthusiasm and | + | Thanks to our instructors' helpful advice, considerate advisors, and great enthusiasm and efforts from all members of the HKUST iGEM 2011 team, we have enjoyed a fantastic summer working with something that we believe will make a difference. <br><br> |
| - | You may visit our <a href=gallery.html target=_top><font color=white><u>Gallery</u></font></a> to see | + | You may visit our <a href=https://2011.igem.org/Team:HKUST-Hong_Kong/gallery.html target=_top><font color=white><u>Gallery</u></font></a> to see the snapshots of us working in the lab. |
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<p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="green"> | <p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="green"> | ||
| - | <span style="line-height: | + | <span style="line-height:1.8; font-weight:600"> |
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a> | | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a> | | ||
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br> | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br> | ||
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| - | <img style=margin:20px src=https://static.igem.org/mediawiki/2011/d/d6/HKUST_Igem_resources.jpg width=120 height=120 | + | <img style=margin:20px src=https://static.igem.org/mediawiki/2011/d/d6/HKUST_Igem_resources.jpg width=120 height=120 align=left> |
<p align="center" valign="baseline" ><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="#D09C00"> | <p align="center" valign="baseline" ><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="#D09C00"> | ||
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<p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="#D09C00"> | <p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="#D09C00"> | ||
| - | <span style="line-height: | + | <span style="line-height:1.3; font-weight:600"> |
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a><br> | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a><br> | ||
The Team<br> | The Team<br> | ||
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<p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="red"> | <p align="left"><font size="1" face="Verdana, Arial, Helvetica, sans-serif" color="red"> | ||
| - | <span style="line-height: | + | <span style="line-height:1.8; font-weight:600"> |
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a> | | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a> | | ||
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a> | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a> | ||
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<p>Our team aims to interfere with this signalling through introducing a disruptor <i>E. coli</i> into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling.</p><br> | <p>Our team aims to interfere with this signalling through introducing a disruptor <i>E. coli</i> into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling.</p><br> | ||
| - | <p>Along the way, we will also create a new strain of <i>E. coli</i> that utilizes an essential gene (<i>nadE</i>) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.</p><br> | + | <p>Along the way, we will also create a new strain of <i>E. coli</i> that utilizes an essential gene (<i>nadE</i>) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.</p><br><br><br> |
</font> | </font> | ||
</font> | </font> | ||
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<p align="center" valign="baseline"> | <p align="center" valign="baseline"> | ||
| - | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements<font color="#FFF4D0"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements</a><font color="#FFF4D0"> | </font> |
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p> | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p> | ||
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<p align="center" valign="baseline"> | <p align="center" valign="baseline"> | ||
| - | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop<font color="white"> | </font> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a><font color="white"> | </font> |
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p> | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p> | ||
Latest revision as of 16:49, 27 October 2011
Greetings from the 2011 HKUST iGEM Team!
This is the 4th time HKUST has participated in this international synthetic biology competition. |
Overview |
Data Page |
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Acknowledgements |
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Project Abstract It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings suggest that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling. Our team aims to interfere with this signalling through introducing a disruptor E. coli into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling. Along the way, we will also create a new strain of E. coli that utilizes an essential gene (nadE) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol. |
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Our Project Experiments and Results
Strain Construction |
Culture Tests |
Modeling Miscellaneous |
iGEM Resources The Team
iGEM Member List |
Contributions Achievements
Medal Requirements |
BioSafety BioBricks |
Human Practice |
