Team:HIT-Harbin/Notebook

From 2011.igem.org

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*<p>An overnight culture grown at 37°C was inoculated in 2.5% glycocoll SMRS as 2% (37°C) and incubated until the optical density at 600 nm (OD600) was 0.5. Cells were harvested by centrifugation (4,000 ×g for 10 min at 4°C) and washed twice with 1 volume of ice-cold electroporation buffe (1 mM KH<sub>2</sub>PO<sub>4</sub>, 0.5 M saccharose , 1 mM MgCl<sub>2</sub>). Finally, pelleted cell were resuspended in 2ml ice-cold electroporation buffer, divided into aliquots and frozen in an ethanol-dry ice bath. Electrocompetent  L.bulgaricus cells were stored at -80°C.</p>
*<p>An overnight culture grown at 37°C was inoculated in 2.5% glycocoll SMRS as 2% (37°C) and incubated until the optical density at 600 nm (OD600) was 0.5. Cells were harvested by centrifugation (4,000 ×g for 10 min at 4°C) and washed twice with 1 volume of ice-cold electroporation buffe (1 mM KH<sub>2</sub>PO<sub>4</sub>, 0.5 M saccharose , 1 mM MgCl<sub>2</sub>). Finally, pelleted cell were resuspended in 2ml ice-cold electroporation buffer, divided into aliquots and frozen in an ethanol-dry ice bath. Electrocompetent  L.bulgaricus cells were stored at -80°C.</p>
*<p>Electrocompetent cells were thawed on ice, and an 50μl cell suspension was mixed with 1μg of recombinant plasmid DNA. After incubation for 30min on ice, the cells were transferred to an electroporation cuvette with a 0.2cm gap between the elec- trodes. A single pulse of 1.2kV lasting 2.5ms was delivered. The electroporated cells were immediately resuspended in 950μl SMRSMC and incubated for 2h at 37°C, before they were spread on MRS plates containing 2μg/ml erythromycin. Transformants were picked following 24 to 48h of incubation at 37°C.</p>
*<p>Electrocompetent cells were thawed on ice, and an 50μl cell suspension was mixed with 1μg of recombinant plasmid DNA. After incubation for 30min on ice, the cells were transferred to an electroporation cuvette with a 0.2cm gap between the elec- trodes. A single pulse of 1.2kV lasting 2.5ms was delivered. The electroporated cells were immediately resuspended in 950μl SMRSMC and incubated for 2h at 37°C, before they were spread on MRS plates containing 2μg/ml erythromycin. Transformants were picked following 24 to 48h of incubation at 37°C.</p>
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==='''The competent cell of Streptococcus thermophilus'''===
==='''The competent cell of Streptococcus thermophilus'''===
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=='''PCR Reaction'''==
=='''PCR Reaction'''==
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Revision as of 03:12, 6 October 2011

Contents

Weekly Diary


Protocal

The Composition Of Media

LB Medium

Yeast extact 5g

Peptone 10g

NaCl 10g

Agar 1-2%

Distilled water 1000ml pH 7.0

Boil the mixture in autoclave at 121℃ for 30min

Range of applicationb: Escherichia.coli

MRS Medium

Peptone 10g

Beef extract 10g

Yeast extact 5g

K2HPO4 2g

Tween 80 1ml

Diamine citrate 2g

Na-acetate 5g

MgSO4.7H2O 0.5g

MnSO4.H2O 0.25g

Glucose 20g

Agar 1-2%

Distilled water 1000ml Adjust pH to 6.4-6.8

Boil the mixture in autoclave at 115℃ for 20min

Range of application: Lactobacillus bulgaricus

2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2

SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2

Hogg-Jago glucose broth(HJG) medium

Tryptone 30g

Yeast extract 10g

Beef extract 2g

KH2PO4 5g

Glucose 5g

Agar 1.5%

Distilled water 1000ml

Boil the mixture in autoclave at 115℃ for 20min

Range of application: Streptococcus thermophilus

HJGL is Hogg-Jago glucose broth supplemented with 0.5% lactose, whereas HJGLS is HJGL supplemented with 0.4M D-sorbitol

Agar plates were prepared by adding 1.5% (wt/vol) agar to the media

Competent Cell and Electroporation

The competent cell of Lactobacillus bulgaricus

  • An overnight culture grown at 37°C was inoculated in 2.5% glycocoll SMRS as 2% (37°C) and incubated until the optical density at 600 nm (OD600) was 0.5. Cells were harvested by centrifugation (4,000 ×g for 10 min at 4°C) and washed twice with 1 volume of ice-cold electroporation buffe (1 mM KH2PO4, 0.5 M saccharose , 1 mM MgCl2). Finally, pelleted cell were resuspended in 2ml ice-cold electroporation buffer, divided into aliquots and frozen in an ethanol-dry ice bath. Electrocompetent L.bulgaricus cells were stored at -80°C.

  • Electrocompetent cells were thawed on ice, and an 50μl cell suspension was mixed with 1μg of recombinant plasmid DNA. After incubation for 30min on ice, the cells were transferred to an electroporation cuvette with a 0.2cm gap between the elec- trodes. A single pulse of 1.2kV lasting 2.5ms was delivered. The electroporated cells were immediately resuspended in 950μl SMRSMC and incubated for 2h at 37°C, before they were spread on MRS plates containing 2μg/ml erythromycin. Transformants were picked following 24 to 48h of incubation at 37°C.

The competent cell of Streptococcus thermophilus

PCR Reaction


Agarose Gel Electrophoresis


Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)