Team:HIT-Harbin/Notebook

From 2011.igem.org

(Difference between revisions)
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<p>Range of applicationb: Escherichia.coli</p>
<p>Range of applicationb: Escherichia.coli</p>
==='''MRS Medium'''===
==='''MRS Medium'''===
-
<p>Peptone          10g<p>  
+
<p>Peptone          10g</p>  
-
<p>Beef extract      10g<p>
+
<p>Beef extract      10g</p>
-
<p>Yeast extact      5g<p>  
+
<p>Yeast extact      5g</p>  
-
<p>K<sub>2</sub>HPO<sub>4</sub>            2g<p>
+
<p>K<sub>2</sub>HPO<sub>4</sub>            2g</p>
-
<p>Tween 80          1ml<p>
+
<p>Tween 80          1ml</p>
-
<p>Diamine citrate    2g<p>
+
<p>Diamine citrate    2g</p>
-
<p>Na-acetate        5g<p>
+
<p>Na-acetate        5g</p>
-
<p>MgSO<sub>4</sub>.7H<sub>2</sub>O        0.5g<p>
+
<p>MgSO<sub>4</sub>.7H<sub>2</sub>O        0.5g</p>
-
<p>MnSO<sub>4</sub>.H<sub>2</sub>O        0.25g<p>
+
<p>MnSO<sub>4</sub>.H<sub>2</sub>O        0.25g</p>
-
<p>Glucose          20g<p>
+
<p>Glucose          20g</p>
-
<p>Agar              1-2%<p>
+
<p>Agar              1-2%</p>
-
<p>Distilled water 1000ml    Adjust pH to 6.4-6.8<p>  
+
<p>Distilled water 1000ml    Adjust pH to 6.4-6.8</p>  
-
<p>Boil the mixture in autoclave at 115℃ for 20min<p>  
+
<p>Boil the mixture in autoclave at 115℃ for 20min</p>  
-
<p>Range of application: Lactobacillus bulgaricus<p>
+
<p>Range of application: Lactobacillus bulgaricus</p>
-
<p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.<p>  
+
<p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.</p>  
-
<p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.<p>
+
<p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.</p>
==='''Hogg-Jago glucose broth(HJG) medium'''===
==='''Hogg-Jago glucose broth(HJG) medium'''===

Revision as of 03:00, 6 October 2011

Contents

Weekly Diary


Protocal

The Composition Of Media

LB Medium

Yeast extact 5g

Peptone 10g

NaCl 10g

Agar 1-2%

Distilled water 1000ml pH 7.0

Boil the mixture in autoclave at 121℃ for 30min

Range of applicationb: Escherichia.coli

MRS Medium

Peptone 10g

Beef extract 10g

Yeast extact 5g

K2HPO4 2g

Tween 80 1ml

Diamine citrate 2g

Na-acetate 5g

MgSO4.7H2O 0.5g

MnSO4.H2O 0.25g

Glucose 20g

Agar 1-2%

Distilled water 1000ml Adjust pH to 6.4-6.8

Boil the mixture in autoclave at 115℃ for 20min

Range of application: Lactobacillus bulgaricus


2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2.


SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2.

Hogg-Jago glucose broth(HJG) medium

Competent Cell and Electroporation

The competent cell of Lactobacillus bulgaricus

The competent cell of Streptococcus thermophilus

PCR Reaction

Agarose Gel Electrophoresis

Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)