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Team:HIT-Harbin/Notebook
From 2011.igem.org
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='''Protocal'''= | ='''Protocal'''= | ||
<div class="cadre"> | <div class="cadre"> | ||
| + | =='''The Composition Of Media'''== | ||
| + | ==='''LB Medium'''=== | ||
| + | <p>Yeast extact 5g</p> | ||
| + | <p>Peptone 10g</p> | ||
| + | <p>NaCl 10g</p> | ||
| + | <p>Agar 1-2%</p> | ||
| + | <p>Distilled water 1000ml pH 7.0</p> | ||
| + | <p>Boil the mixture in autoclave at 121℃ for 30min </p> | ||
| + | <p>Range of applicationb: Escherichia.coli</p> | ||
| + | ==='''MRS Medium'''=== | ||
| + | <p>Peptone 10g<p> | ||
| + | <p>Beef extract 10g<p> | ||
| + | <p>Yeast extact 5g<p> | ||
| + | <p>K<sub>2</sub>HPO<sub>4</sub> 2g<p> | ||
| + | <p>Tween 80 1ml<p> | ||
| + | <p>Diamine citrate 2g<p> | ||
| + | <p>Na-acetate 5g<p> | ||
| + | <p>MgSO<sub>4</sub>.7H<sub>2</sub>O 0.5g<p> | ||
| + | <p>MnSO<sub>4</sub>.H<sub>2</sub>O 0.25g<p> | ||
| + | <p>Glucose 20g<p> | ||
| + | <p>Agar 1-2%<p> | ||
| + | <p>Distilled water 1000ml Adjust pH to 6.4-6.8<p> | ||
| + | <p>Boil the mixture in autoclave at 115℃ for 20min<p> | ||
| + | <p>Range of application: Lactobacillus bulgaricus<p> | ||
| + | |||
| + | |||
| + | <p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.<p> | ||
| + | |||
| + | |||
| + | <p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl<sub>2</sub>,20mmol/L CaCl<sub>2</sub>.<p> | ||
| + | ==='''Hogg-Jago glucose broth(HJG) medium'''=== | ||
| + | |||
| + | =='''Competent Cell and Electroporation'''== | ||
| + | ==='''The competent cell of Lactobacillus bulgaricus'''=== | ||
| + | ==='''The competent cell of Streptococcus thermophilus'''=== | ||
| + | =='''PCR Reaction'''== | ||
| + | =='''Agarose Gel Electrophoresis'''== | ||
| + | =='''Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)'''== | ||
| + | |||
</div> | </div> | ||
{{:Team:HIT-Harbin/footer}} | {{:Team:HIT-Harbin/footer}} | ||
Revision as of 02:59, 6 October 2011
Contents |
Weekly Diary
Protocal
The Composition Of Media
LB Medium
Yeast extact 5g
Peptone 10g
NaCl 10g
Agar 1-2%
Distilled water 1000ml pH 7.0
Boil the mixture in autoclave at 121℃ for 30min
Range of applicationb: Escherichia.coli
MRS Medium
Peptone 10g<p> <p>Beef extract 10g<p> <p>Yeast extact 5g<p> <p>K2HPO4 2g<p> <p>Tween 80 1ml<p> <p>Diamine citrate 2g<p> <p>Na-acetate 5g<p> <p>MgSO4.7H2O 0.5g<p> <p>MnSO4.H2O 0.25g<p> <p>Glucose 20g<p> <p>Agar 1-2%<p> <p>Distilled water 1000ml Adjust pH to 6.4-6.8<p> <p>Boil the mixture in autoclave at 115℃ for 20min<p> <p>Range of application: Lactobacillus bulgaricus<p> <p>2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2.<p> <p>SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl2,20mmol/L CaCl2.<p>
Hogg-Jago glucose broth(HJG) medium
Competent Cell and Electroporation
The competent cell of Lactobacillus bulgaricus
The competent cell of Streptococcus thermophilus
PCR Reaction
Agarose Gel Electrophoresis
Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)
</div>
