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Team:Groningen/project notebook/6 June 2011
From 2011.igem.org
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Week 23 | Week 23 | ||
*holidays | *holidays | ||
| + | '''Christoph''' | ||
| + | Cloned reversed PLasB to pSB1A3+DT | ||
| + | Obtained PCR products for all cI variants | ||
| + | |||
{{FooterGroningen2011}} | {{FooterGroningen2011}} | ||
Latest revision as of 23:35, 21 September 2011
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Joyce
Plasmid Prep of the biobricks: DT, cI, LasRLVA, RFP,PBADaraC, RFP DT, PlasB, RBS-GFP-DT
ND1000- nanodrop measurements:
DT: 53.9 ng/μl
cI: 64.1 ng/μl
LasRLVA: 43.3 ng/μl
RFP: 32.9 ng/μl
PBADaraC: 53.2 ng/μl
RFP DT: 53.3 ng/μl
PlasB: 44.8 ng/μl
RBS-GFP-DT: 52.5 ng/μl
Samples were checked again with restriction analysis.
Digestion mix:
14μl FD buffer
3.5μl EcoRI
3.5μl PstI
49μl MQ water
10μl mix+ 10μl DNA sample
Analyse on a 1% TBE agarose gel
Samples look alright, DT, cI, LasRLVA, RFP, PBADaraC, RFP DT, PlasB, RBS-GFP-DT were send for sequencing
Jakub:
Week 23
- holidays
Christoph Cloned reversed PLasB to pSB1A3+DT Obtained PCR products for all cI variants
