"
Team:Groningen/project notebook/4 July 2011
From 2011.igem.org
(Difference between revisions)
| Line 19: | Line 19: | ||
Week 27: | Week 27: | ||
*Cloning LasR variants into plasmids with reverse double terminator and LasB promoter - first try. | *Cloning LasR variants into plasmids with reverse double terminator and LasB promoter - first try. | ||
| + | |||
| + | '''Christoph''' | ||
| + | *Cloning the last cI variants into the plasmid with DT, because some sequences seemd not alright. | ||
{{FooterGroningen2011}} | {{FooterGroningen2011}} | ||
Revision as of 23:45, 21 September 2011
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Joyce
PCR of HybB promotor, PcI, PlasI, LasR-LVA, cI LVA.
PCR protocol:
Denaturation: 94 °C 5 minutes
Cycle 30×
Denaturation: 94 °C 30 seconds
Annealing: 30 seconds 60 degrees
Extension: 2 minutes
Final extension: 7 minutes
Store infinite at 4 °C
Analyze on 1% agarose gel
Work on Human Practices
Jakub
Week 27:
- Cloning LasR variants into plasmids with reverse double terminator and LasB promoter - first try.
Christoph
- Cloning the last cI variants into the plasmid with DT, because some sequences seemd not alright.
