"
Team:Groningen/project notebook/26 October 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "{{HeaderGroningen2011}} {{notebookgroningen}} '''Olesja''' <br> inoculation of 5 ml LB (with Kanamycin) <br> -> four colonies per transformation (see day before) {{FooterGronin...") |
|||
| Line 1: | Line 1: | ||
{{HeaderGroningen2011}} {{notebookgroningen}} | {{HeaderGroningen2011}} {{notebookgroningen}} | ||
'''Olesja''' <br> | '''Olesja''' <br> | ||
| + | FACS measurement (0, 6 h) of the following contructs: | ||
| + | * Prm_cI (loop 1-11) + Pbad/araC_cI_Prm_GFP <br> | ||
| + | un-induced and induced with 1 % arabinose | ||
| + | <br> | ||
| + | <br> | ||
inoculation of 5 ml LB (with Kanamycin) <br> | inoculation of 5 ml LB (with Kanamycin) <br> | ||
-> four colonies per transformation (see day before) | -> four colonies per transformation (see day before) | ||
{{FooterGroningen2011}} | {{FooterGroningen2011}} | ||
Latest revision as of 21:23, 28 October 2011
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Olesja
FACS measurement (0, 6 h) of the following contructs:
- Prm_cI (loop 1-11) + Pbad/araC_cI_Prm_GFP
un-induced and induced with 1 % arabinose
inoculation of 5 ml LB (with Kanamycin)
-> four colonies per transformation (see day before)
