Team:Groningen/project notebook/25 October 2011

From 2011.igem.org

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'''Olesja''' <br>
'''Olesja''' <br>
*inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement
*inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement
 +
<br>
 +
<br>
*gel-extraction of Pbad/AraC_cI_Prm_GFP construct <br>
*gel-extraction of Pbad/AraC_cI_Prm_GFP construct <br>
cloning into plasmid containing cI-loop construct <br>
cloning into plasmid containing cI-loop construct <br>

Revision as of 21:25, 28 October 2011


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Olesja

  • inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement



  • gel-extraction of Pbad/AraC_cI_Prm_GFP construct

cloning into plasmid containing cI-loop construct
transformation of competent cells with ligated construct