Team:Groningen/project notebook/25 October 2011
From 2011.igem.org
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'''Olesja''' <br> | '''Olesja''' <br> | ||
*inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement | *inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement | ||
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*gel-extraction of Pbad/AraC_cI_Prm_GFP construct <br> | *gel-extraction of Pbad/AraC_cI_Prm_GFP construct <br> | ||
cloning into plasmid containing cI-loop construct <br> | cloning into plasmid containing cI-loop construct <br> |
Revision as of 21:25, 28 October 2011
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Olesja
- inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement
- gel-extraction of Pbad/AraC_cI_Prm_GFP construct
cloning into plasmid containing cI-loop construct
transformation of competent cells with ligated construct