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Team:Groningen/project notebook/25 October 2011
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'''Olesja''' <br> | '''Olesja''' <br> | ||
| - | gel-extraction of Pbad/AraC_cI_Prm_GFP construct <br> | + | *inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement |
| + | *gel-extraction of Pbad/AraC_cI_Prm_GFP construct <br> | ||
cloning into plasmid containing cI-loop construct <br> | cloning into plasmid containing cI-loop construct <br> | ||
transformation of competent cells with ligated construct | transformation of competent cells with ligated construct | ||
Revision as of 21:19, 28 October 2011
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Olesja
- inoculation of M9 media (with CA and A/K) with cotransformants for FACS measurement
- gel-extraction of Pbad/AraC_cI_Prm_GFP construct
cloning into plasmid containing cI-loop construct
transformation of competent cells with ligated construct
