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Team:Groningen/project notebook/18 September 2011
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'''Olesja''' <br> | '''Olesja''' <br> | ||
| - | flourimeter measurement of following contstructs (in plate reader) | + | flourimeter measurement of following contstructs (in plate reader)<br> |
| + | measurement over time, 50 cycles of 15 min | ||
*PBad_cI + Prm_GFP (potentially wrong construct)<br> | *PBad_cI + Prm_GFP (potentially wrong construct)<br> | ||
induced with different arabinose concentrations (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 %) | induced with different arabinose concentrations (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 %) | ||
Latest revision as of 11:12, 20 September 2011
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Olesja
flourimeter measurement of following contstructs (in plate reader)
measurement over time, 50 cycles of 15 min
- PBad_cI + Prm_GFP (potentially wrong construct)
induced with different arabinose concentrations (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 %)
- PlacI_LasR + PlasB_RFP
induced with different IPTG concentrations (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.75, 1.0, 10, 50 mM)
- PBad_GFP
induced with different arabinose concentrations (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 %)
