Team:Groningen/project notebook/13 June 2011
From 2011.igem.org
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Week 24: | Week 24: | ||
*cloning double terminator into chloamphenicol, ampicilin, kanamycin and tetracyclin backbones | *cloning double terminator into chloamphenicol, ampicilin, kanamycin and tetracyclin backbones | ||
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+ | '''Christoph''' | ||
+ | *Repeated cloning of reversed PLasB into pSB1A3+DT. | ||
+ | *Cloned the cI variants into pSB1K3+rev.Terminator | ||
{{FooterGroningen2011}} | {{FooterGroningen2011}} |
Revision as of 23:39, 21 September 2011
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Joyce
Plasmid prep of the overnight cultures
ND1000-nanodrop measurements: DNA concentrations are between 30 and 60 ng/μl
Inoculating 2 erlenmeyers of 200ML , 1 for making competentcells DH5alpha and other one for TOP10 cells
Also TOP10cells will be send to the iGEM Wageningen team.
Also preheat other 2 200ml erlenmeyers
Restriction analysis:
Digestion test:
2μl FD buffer
0.5μl EcoRI
0.5μl SpeI
8μl MQ water
10μl + 10μl sample
Digestion mix:
30μl FD buffer
7.5μl EcoRI
7.5μl PstI
120μl MW water
Digestion mix:
30μl FD buffer
7.5μl XbaI
7.5μl SpeI
120μl MW water
Work in Clone manager: check primers PhybB again
Jakub
Week 24:
- cloning double terminator into chloamphenicol, ampicilin, kanamycin and tetracyclin backbones
Christoph
- Repeated cloning of reversed PLasB into pSB1A3+DT.
- Cloned the cI variants into pSB1K3+rev.Terminator