Team:Groningen/project notebook/13 June 2011

From 2011.igem.org

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Week 24:
Week 24:
*cloning double terminator into chloamphenicol, ampicilin, kanamycin and tetracyclin backbones
*cloning double terminator into chloamphenicol, ampicilin, kanamycin and tetracyclin backbones
 +
 +
'''Christoph'''
 +
*Repeated cloning of reversed PLasB into pSB1A3+DT.
 +
*Cloned the cI variants into pSB1K3+rev.Terminator
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Revision as of 23:39, 21 September 2011


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Joyce


Plasmid prep of the overnight cultures
ND1000-nanodrop measurements: DNA concentrations are between 30 and 60 ng/μl

Inoculating 2 erlenmeyers of 200ML , 1 for making competentcells DH5alpha and other one for TOP10 cells
Also TOP10cells will be send to the iGEM Wageningen team.
Also preheat other 2 200ml erlenmeyers

Restriction analysis:
Digestion test:
2μl FD buffer
0.5μl EcoRI
0.5μl SpeI
8μl MQ water
10μl + 10μl sample

Digestion mix:
30μl FD buffer
7.5μl EcoRI
7.5μl PstI
120μl MW water

Digestion mix:
30μl FD buffer
7.5μl XbaI
7.5μl SpeI
120μl MW water

Work in Clone manager: check primers PhybB again


Jakub


Week 24:

  • cloning double terminator into chloamphenicol, ampicilin, kanamycin and tetracyclin backbones

Christoph

  • Repeated cloning of reversed PLasB into pSB1A3+DT.
  • Cloned the cI variants into pSB1K3+rev.Terminator