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Team:Grinnell CN/Recipes - Revision history
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2011-09-27T04:14:27Z
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http://2011.igem.org/wiki/index.php?title=Team:Grinnell_CN/Recipes&diff=181980&oldid=prev
Aaring: Created page with "{{:Team:Grinnell/Template/Home}} <html> <head> <style type="text/css"> th{ width:100px; } td{ text-align:center; } </style> </head <body> <h1>Recipes</h1> <h2><a name="LB">..."
2011-09-27T03:57:05Z
<p>Created page with "{{:Team:Grinnell/Template/Home}} <html> <head> <style type="text/css"> th{ width:100px; } td{ text-align:center; } </style> </head <body> <h1>Recipes</h1> <h2><a name="LB">..."</p>
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<h1>Recipes</h1><br />
<br />
<br />
<h2><a name="LB"></a>L Broth</h2><br />
For 1L:<ul><br />
<li>10g Tryptone</li><br />
<li>5g Yeast extract</li><br />
<li>5g NaCl</li></ul><br />
<br />
<h2><a name="PYE"></a>PYE Broth</h2><br />
For 1L:<ul><br />
<li>2g Bacto peptone</li><br />
<li>1g Yeast extract</li><br />
<li>1mL 1M MgSO<sub>4</sub>&sdot;7H<sub>2</sub>O</li><br />
<li>1mL 0.5M CaCl<sub>2</sub></li></ul><br />
<br />
<h2><a name="Agar"></a>Agar Plates</h2><br />
Add 15g Bacto-agar to 1L of liquid media before autoclaving.<br />
<br />
<h2><a name="Anti"></a>Antibiotics Kitchen</h2><br />
<table><br />
<tr><br />
<th>Antibiotic</th><br />
<th>Color Code</th><br />
<th>Stock (mg/mL)</th><br />
<th>LB Plate Conc (&mu;g/mL)</th><br />
<th>PYE Plate Conc (&mu;g/mL)</th><br />
</tr><br />
<tr><br />
<td style="text-align:left">Ampicillin (amp)</td><br />
<td>Red</td><br />
<td>50</td><br />
<td>100</td><br />
<td>50</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Carbenicillin (carb)</td><br />
<td>Red</td><br />
<td>50</td><br />
<td>50</td><br />
<td>50</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Chloramphenicol (chlor)</td><br />
<td>2 Orange</td><br />
<td>5 (in EtOH)</td><br />
<td>30</td><br />
<td>1</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Kanamycin (kan)</td><br />
<td>blue</td><br />
<td>50</td><br />
<td>50</td><br />
<td>25</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Nalidixic acid (nal)</td><br />
<td>Green</td><br />
<td>10</td><br />
<td>20</td><br />
<td>20</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Tetracycline (tet)</td><br />
<td>Black</td><br />
<td>2 (in EtOH)</td><br />
<td>12</td><br />
<td>&ndash;</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Oxytetracycline (oxytet)</td><br />
<td>Black</td><br />
<td>2 (in EtOH)</td><br />
<td>&minus;</td><br />
<td>2</td><br />
</tr><br />
<tr><br />
<td style="text-align:left">Spectinomycin (spec)</td><br />
<td>Purple</td><br />
<td>20</td><br />
<td>50</td><br />
<td>100</td><br />
</tr><br />
</table><br />
<br/><b>Note</b>: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures).<br />
<br />
<h2><a name="freezerStocks"></a>Freezer Stocks</h2><br />
To an appropriate tube add:<br />
<ul><br />
<li>700&mu;L overnight liquid culture</li><br />
<li>300&mu;L 50% glycerol sol'n</li><br />
</ul><br />
Then place in storage at -80&deg;C.<br />
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