"
Team:Grinnell CN/Recipes
From 2011.igem.org
(Difference between revisions)
(Created page with "{{:Team:Grinnell/Template/Home}} <html> <head> <style type="text/css"> th{ width:100px; } td{ text-align:center; } </style> </head <body> <h1>Recipes</h1> <h2><a name="LB">...") |
|||
| Line 1: | Line 1: | ||
| - | {{:Team: | + | {{:Team:Grinnell_CN/Template/Home}} |
<html> | <html> | ||
Latest revision as of 04:14, 27 September 2011
Recipes
L Broth
For 1L:- 10g Tryptone
- 5g Yeast extract
- 5g NaCl
PYE Broth
For 1L:- 2g Bacto peptone
- 1g Yeast extract
- 1mL 1M MgSO4⋅7H2O
- 1mL 0.5M CaCl2
Agar Plates
Add 15g Bacto-agar to 1L of liquid media before autoclaving.Antibiotics Kitchen
| Antibiotic | Color Code | Stock (mg/mL) | LB Plate Conc (μg/mL) | PYE Plate Conc (μg/mL) |
|---|---|---|---|---|
| Ampicillin (amp) | Red | 50 | 100 | 50 |
| Carbenicillin (carb) | Red | 50 | 50 | 50 |
| Chloramphenicol (chlor) | 2 Orange | 5 (in EtOH) | 30 | 1 |
| Kanamycin (kan) | blue | 50 | 50 | 25 |
| Nalidixic acid (nal) | Green | 10 | 20 | 20 |
| Tetracycline (tet) | Black | 2 (in EtOH) | 12 | – |
| Oxytetracycline (oxytet) | Black | 2 (in EtOH) | − | 2 |
| Spectinomycin (spec) | Purple | 20 | 50 | 100 |
Note: for liquid cultures in PYE broth, use half the concentration that would be used for a PYE plate (LB remains the same for liquid and sold cultures).
Freezer Stocks
To an appropriate tube add:- 700μL overnight liquid culture
- 300μL 50% glycerol sol'n
