Team:Greenfield IN-Rihm-HS/Notebook

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| style="width:88px;"  | [[Team:Greenfield_IN-Rihm-HS/Team|Team]]
| style="width:88px;"  | [[Team:Greenfield_IN-Rihm-HS/Team|Team]]
| class="left" | [[Team:Greenfield_IN-Rihm-HS/Project|Project]]
| class="left" | [[Team:Greenfield_IN-Rihm-HS/Project|Project]]
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| class="left" style="width:100px;" | [[Team:Greenfield_IN-Rihm-HS/Modeling|Modeling]]
 
| class="left" style="width:110px;" | [[Team:Greenfield_IN-Rihm-HS/Notebook|Notebook]]
| class="left" style="width:110px;" | [[Team:Greenfield_IN-Rihm-HS/Notebook|Notebook]]
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| class="left" style="width:110px;" | [[Team:Greenfield_IN-Rihm-HS/Parts|Parts]]
| class="left" style="width:100px;" | [[Team:Greenfield_IN-Rihm-HS/Safety|Safety]]
| class="left" style="width:100px;" | [[Team:Greenfield_IN-Rihm-HS/Safety|Safety]]
| class="left" style="width:115px;" | [[Team:Greenfield_IN-Rihm-HS/Attributions|Attributions]]
| class="left" style="width:115px;" | [[Team:Greenfield_IN-Rihm-HS/Attributions|Attributions]]
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''Our project this year is to design, create, and test a working cadmium water tester. We are going to do this by altering the existing biochemical pathway that Yeast has to recognize the cadmium, and then create our own code that will make it fluoresce red. When we originally had the idea for a water tester, we looked for metals, pesticides, and other harmful substances that are a problem in water supplies. Through much research, we found that cadmium is often a byproduct of many industrial processes, and is often leaked or exposed to the environment. Also, yeast recognizes the presence of cadmium already, so we figured it would be much more simple to alter an existing pathway than to create an entirely new one. We hope through research and experimentation that we are able to make cadmium much more detectable in the environment.''
 
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|[[Image:Greenfield_IN-Rihm-HS_team.png|right| 250px |Your team picture]]
 
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|align="center"|[[Team:Greenfield_IN-Rihm-HS | Greenfield-Central High School
 
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Team Rihm]]
 
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{|align="justify"
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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|''Our project this year is to design, create, and test a working cadmium water tester. We are going to do this by altering the existing biochemical pathway that Yeast has to recognize the cadmium, and then create our own code that will make it fluoresce red. When we originally had the idea for a water tester, we looked for metals, pesticides, and other harmful substances that are a problem in water supplies. Through much research, we found that cadmium is often a byproduct of many industrial processes, and is often leaked or exposed to the environment. Also, yeast recognizes the presence of cadmium already, so we figured it would be much more simple to alter an existing pathway than to create an entirely new one. We hope through research and experimentation that we are able to make cadmium much more detectable in the environment.
|[[Image:iGem logo.png| 200px |right]]
|[[Image:iGem logo.png| 200px |right]]
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''Our project this year is to design, create, and test a working cadmium water tester. We are going to do this by altering the existing biochemical pathway that Yeast has to recognize the cadmium, and then create our own code that will make it fluoresce red. When we originally had the idea for a water tester, we looked for metals, pesticides, and other harmful substances that are a problem in water supplies. Through much research, we found that cadmium is often a byproduct of many industrial processes, and is often leaked or exposed to the environment. Also, yeast recognizes the presence of cadmium already, so we figured it would be much more simple to alter an existing pathway than to create an entirely new one. We hope through research and experimentation that we are able to make cadmium much more detectable in the environment.''
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''
|[[Image:Greenfield_IN-Rihm-HS_team.png|right| 250px |Your team picture]]
|[[Image:Greenfield_IN-Rihm-HS_team.png|right| 250px |Your team picture]]
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==Calender/Notebook==
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<html><head><title>Calendar2011</title></head><body onload='window.focus();'>
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<center><H2><font size="2" face="Verdana">April 2011
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'''April 13th:'''
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</font></H2></center><TABLE width="100%" border=1 bordercolordark="#000000" bordercolorlight="#FFFFFF" cellpadding="5">
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<TBODY><TR><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Officially met for the first time and discussed ideas for our project. Some of the original ideas included a strain of yeast that acted as a water tester, a strain of yeast that emitted a cinnamon scent, and a strain of yeast that reverses the oxidation of copper.
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Mon</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Tues</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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'''April 20th:'''
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Wed</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Thur</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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After we read an article on how yeast copes with toxic metals (Wysocki & Tamas, 2010), we made the final decision of making a water tester. We discussed what metals we wanted to detect in an environment, and we decided on copper, becase yeast already has a promoter associated with copper, Cup1.
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Fri</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Sat</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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'''April 23th:'''
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Sun</font></TH></TR><TR>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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We met early in the morning and discussed how to actually make our plasmid. We read about and decided to use Ura3 as our building block, and that we were going to put our plasmid in Ura3 (Mumberg, Muller, & Funk, 1995). We learned about the Gibson Method, a new system of putting together DNA parts instead of using restrictive enzymes. We also learned the order of our part: Promoter, Kozak Sequence, Space, Protein Coding Region, and then Terminator.
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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'''April 27th:'''
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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We changed our project goal, and decided to use Cadmium after reading that cadmium pollution is a much bigger problem than copper pollution, and has a greater detrimental effect on the human body.
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">1</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">2</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">3</font><br><br><br><br></TD>
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'''Citations:'''
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">4</font><br><br><br><br></TD>
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Jeyaprakash, A., Welch J.W., Fogel, S. (1991) Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast. ''Molecular and General Genet.'' 363-368.
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">5</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD>
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Mumberg, D., Muller, R., & Funk, M. (1995) Yeast vectors for the contolled expression of heterologous proteins in different genetic backgrounds. ''Elsevier Science B.V.'' 119-122.
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">7</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">8</font><br><br><br><br></TD>
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Wysocki, R. & Tamas, M.J. (2010) How Saccharomyces cervisiae copes with toxic metals and metalloids. ''Federation of European Microbiology Societies Microbiology Review'' 925-951.
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">9</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">10</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">11</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">12</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/April 13, 2011">13</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">14</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">15</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">16</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">17</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">18</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">19</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/April 20, 2011">20</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">21</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">22</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">23</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">24</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">25</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">26</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/April 27, 2011">27</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">28</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">29</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">30</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br></TD>
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</TR></TBODY></TABLE><br>
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<center><H2><font size="2" face="Verdana">May 2011
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</font></H2></center><TABLE width="100%" border=1 bordercolordark="#000000" bordercolorlight="#FFFFFF" cellpadding="5">
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<TBODY><TR><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Mon</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Tues</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Wed</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Thur</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Fri</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Sat</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Sun</font></TH></TR><TR>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">1</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">2</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">3</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/May 4, 2011">4</a></font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">5</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">7</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">8</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">9</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">10</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/May 11, 2011">11</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">12</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">13</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">14</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">15</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">16</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">17</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">18</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">19</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">20</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">21</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">22</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">23</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">24</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/May 25, 2011">25</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">26</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">27</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">28</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">29</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">30</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/May 31, 2011">31</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br></TD>
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</TR></TBODY></TABLE><br>
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<center><H2><font size="2" face="Verdana">June 2011
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</font></H2></center><TABLE width="100%" border=1 bordercolordark="#000000" bordercolorlight="#FFFFFF" cellpadding="5">
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<TBODY><TR><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Mon</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Tues</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Wed</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Thur</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Fri</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Sat</font></TH><TH valign=center align=middle width="14%" bgcolor="#000000"><font size="1" color="#FFFFFF" face="Verdana">
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Sun</font></TH></TR><TR>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" >&nbsp;<br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 1, 2011">1</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 2, 2011">2</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 3, 2011">3</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">4</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">5</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 6, 2011">6</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 7, 2011">7</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 8, 2011">8</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 9, 2011">9</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 10, 2011">10</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">11</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">12</font><br><br><br><br></TD>
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</tr><tr>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 13, 2011">13</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 14, 2011">14</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 15, 2011">15</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 16, 2011">16</font><br><br><br><br></TD>
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<TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">
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<a href="https://2011.igem.org/Team:Greenfield_IN-Rihm-HS/June 17, 2011">17</font><br><br><br><br></TD>
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Latest revision as of 02:32, 24 June 2011



Our project this year is to design, create, and test a working cadmium water tester. We are going to do this by altering the existing biochemical pathway that Yeast has to recognize the cadmium, and then create our own code that will make it fluoresce red. When we originally had the idea for a water tester, we looked for metals, pesticides, and other harmful substances that are a problem in water supplies. Through much research, we found that cadmium is often a byproduct of many industrial processes, and is often leaked or exposed to the environment. Also, yeast recognizes the presence of cadmium already, so we figured it would be much more simple to alter an existing pathway than to create an entirely new one. We hope through research and experimentation that we are able to make cadmium much more detectable in the environment.
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Greenfield-Central High School Team Rihm



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