Team:Freiburg/SampleData

From 2011.igem.org

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(Our Favorite New Parts)
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;'''1.A)''' Exposure of bacterial culture to red light (705 nm)
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;'''1.A)''' Exposure of bacterial culture to red light (650 nm)
:Red light inducible promoter leads to expression of His-tagged protein of interest.
:Red light inducible promoter leads to expression of His-tagged protein of interest.
<br>
<br>
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;'''1.B)'''Exposure of bacterial culture to blue light (470nm)
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;'''1.B)''' Exposure of bacterial culture to blue light (470nm)
:Blue light leads to a conformational change of the blue light receptor (LovTAP) and the expression of a His-tagged protein.
:Blue light leads to a conformational change of the blue light receptor (LovTAP) and the expression of a His-tagged protein.
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3. [http://partsregistry.org/Part:BBa_K608404 BBa_K608404] '''IPTG-inducible Promoter with plastic binding domain-tagged GFP'''  
3. [http://partsregistry.org/Part:BBa_K608404 BBa_K608404] '''IPTG-inducible Promoter with plastic binding domain-tagged GFP'''  
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The plastic binding domain (pbd) enables the isolation of the Precipitator-His-tagged-protein-complex. The pbd is a small amino acid sequence that can bind plastic, probably due to nonspecific hydrophobic interactions. As hydrophobic proteins can cause problems in E.coli it should be expressed by an inducible promoter. To test the binding interactions of the plastic binding domain it was fused to GFP via a short linker. In our "lab in a cell" model the pbd is part of the Precipitator.
'''Also new parts'''  
'''Also new parts'''  
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[http://partsregistry.org/Part:BBa_K608101 BBa_K608101]
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for the '''green light receptor system'''
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'''CcaR''', green light response regulator
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[http://partsregistry.org/Part:BBa_K608102 BBa_K608102]
[http://partsregistry.org/Part:BBa_K608102 BBa_K608102]
'''CcaS''', green light receptor
'''CcaS''', green light receptor
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CcaS is a cyanobacteriochrome which functions as the receptor for green light.
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After light of the wavelength of 532 nm is exposed to the CcaS receptor, it changes its conformation undergoing autophosphorylation. The phosphate group is transfered to the response regulator CcaR. Once phosphorylated, CcaR can bind to the specific promoter region of cpcG and activate gene expression.
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[http://partsregistry.org/Part:BBa_K608101 BBa_K608101]
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'''CcaR''', green light response regulator
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CcaR is the response regulator of the green light receptor CcaS.
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It belongs to the family of OmpR regulator class. CcaR consists of an N-terminal receiver domain that can be
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phosphorylated by CcaS and a C-terminal DNA-binding domain that binds directly to the promoter region of cpcG2.
== '''Pre-existing Parts''' ==
== '''Pre-existing Parts''' ==
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==<span style="color:orange;">Lysis cassette</span>==
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<span style="color:orange;">Lysis cassette</span>
'''The Concept'''<br>
'''The Concept'''<br>
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== '''We've Also Characterized the Following Parts''' ==
== '''We've Also Characterized the Following Parts''' ==
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Here we cloned different promoters with different RBS for individual gene expression.

Latest revision as of 03:52, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!