Team:Freiburg/Results

From 2011.igem.org

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(red light receptor)
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==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
The handling with the LOV domain and TrpR domain was very difficult.<br/>
The handling with the LOV domain and TrpR domain was very difficult.<br/>
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On the one hand it was difficlut to digest the parts, because there was a SpeI restriction site in the tryptophan repressible promoter region. So we first had to mutate SpeI into NehI restriction site to digest our parts without digesting the promotor. In the beginning we also tried the Gibson assembly to avoid mutating the restriction site. We had trouble to amplify the Lov domain. Reasons might be the size, wrong primer design or the high annealing temperature. The 3A-assembly with the mutated parts wasn´t successful either. We only cloned the Not-Gate in the pSB1C3 vector. We tried this experiment with different parameters but we weren´t successful.
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On the one hand it was difficlut to digest the parts, because there was a SpeI restriction site in the tryptophan repressible promoter region. So we first had to mutate SpeI into NehI restriction site to digest our parts without digesting the promotor. In the beginning we also tried the Gibson assembly. Primer design was quite difficult because of the lenght, the usually very high annealing temperature and unspecifity of the sequence combined to the mutations we had to include.  These hassel leaded to trouble to amplify the Lov domain.The 3A-assembly with the mutated parts wasn´t successful either. We only cloned the Not-Gate in the pSB1C3 vector. We tried this experiment with different parameters but we weren´t successful.
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==

Revision as of 23:05, 21 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!