Team:Freiburg/Results

From 2011.igem.org

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Precipitator
Precipitator
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The Precipitator is a new artificially designed LRR protein. It is meant as protein that binds Nickel ions with Histidines grouped on its surface. The bound Nickel can then precipitate His-tagged proteins. In our Lab in a Cell it should function as an adaptor between the plastic surface of pipettes and the His-tagged protein. Please look at our detailed description of the design layout in our modeling section.
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The Precipitator is a new artificially designed LRR protein. It is meant as protein that binds Nickel ions with Histidines grouped on its surface. The bound Nickel can then precipitate His-tagged proteins. In our Lab in a Cell it should function as an adaptor between the plastic surface of pipettes and the His-tagged protein. Please look at our detailed description of the design layout in our modeling section. The sequence was synthesised and cloned into the iGEm vector. The sequence was partially confirmed by sequencing.
[http://partsregistry.org/Part:BBa_K608407 BBa_K608407]
[http://partsregistry.org/Part:BBa_K608407 BBa_K608407]

Revision as of 22:26, 20 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Parts submitted to the registry

All our results are documented in our notebook and summarized in the partsregistry. Here we shortly describe what happened to give you an idea about the current status of the different subprojects. Please follow the links for more information.

Precipitator

BBa_K608404 IPTG-inducible Promoter with plastic binding domain-tagged GFP

BBa_K608406 Precipitator

The Precipitator is a new artificially designed LRR protein. It is meant as protein that binds Nickel ions with Histidines grouped on its surface. The bound Nickel can then precipitate His-tagged proteins. In our Lab in a Cell it should function as an adaptor between the plastic surface of pipettes and the His-tagged protein. Please look at our detailed description of the design layout in our modeling section. The sequence was synthesised and cloned into the iGEm vector. The sequence was partially confirmed by sequencing.

BBa_K608407 Precipitator fused with GST tag

The Precipitator was fused with the C-terminus of our GST tag in order to extract and further test the construct for its Nickel binding affinity. We successfully cloned it together using the Gibson Assembly and then subsequently pasted it into the iGEM vector. The sequence was partially confirmed by sequencing.

BBa_K608408 GST-tag

The GST-tag was PCR amplified from a pGEX vector with overhang primers including the iGEM restriction sites and then pasted into the iGEM vector. To verify the functionality of the construct we cloned it before a GFP sequence and expressed it with an IPTG inducible vector. Results see partsregistry page.

Green light receptor

BBa_K608101 CcaR, green light response regulator

BBa_K608102 CcaS, green light receptor

BBa_K608151 translational unit of pcyA

Lysis cassette

BBa_K608351 correct temperatursesitive promotor

BBa_K608352 Bacteriophage Lysis Cassette with RBS

commons

BBa_K608002 strong Promotor and strong RBS

BBa_K608003 strong Promotor , medium RBS

BBa_K608004 strong Promotor , weak RBS

BBa_K608005 medium Promotor , strong RBS

BBa_K608006 medium Promotor , medium RBS

BBa_K608007 medium Promotor , weak RBS

BBa_K608008 constitutive strong promotor with medium RBS and GFP

BBa_K608009 Strong promotor with weak RBS and GFP

BBa_K608010 Medium promotor with strong RBS and GFP

BBa_K608011 Medium promotor with medium RBS and GFP

BBa_K608012 Medium promotor with weak RBS and GFP

BBa_K608013 strong Promotor with strong RBS and RFP

BBa_K608014 PR with RFP

BBa_K608015 PR with RFP

BBa_K608016 PR with RFP

BBa_K608017 PR with RFP

BBa_K608018 PR with RFP


                       

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