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List of "broken" parts

Lysis device

[http://partsregistry.org/Part:BBa_K098995 K098995]: 6 nt missing between RBS and the cI repressor possibly leading to a diminished expression (not tested.)

We are trying to insert the 6 nt and test the part again.

[http://partsregistry.org/Part:BBa_K112022 K112022]: Sequencing with VF primer did not work, digest shows unwanted pattern

[http://partsregistry.org/Part:BBa_K124014 K124014]: has several issues:

1. it’s not the holin 105 gene but it resembles part [http://partsregistry.org/wiki/index.php/Part:BBa_K124017 K124017]
2. There are two AA exchange mutations in the holin 105 gene compared to the sequence submitted for [http://partsregistry.org/wiki/index.php/Part:BBa_K124017 K124017].
3. The wrong prefix was used what will finally lead to a RBS-ATG distance of 8 instead of 6 nucleotides

The overall structure of the submitted sequence is likely the amplified original lysis gen cluster of the bacteriophage lambda without any modifications. We try to utilize it for our experiments, maybe we need to change the prefix, doing it by simply amplifying it with the correct prefix and re-clone it into pSB1C3

Light receptors

[http://partsregistry.org/Part:BBa_M30109 M30109]: The composite (red light receptor) is simply not what it should be.

Only the gene for chp8 is present in the plasmid, as already outlined in the [http://partsregistry.org/Part:BBa_M30109:Experience experience] page.

[http://partsregistry.org/Part:BBa_I15010 I15010]: should have been the original chp8 gen, but sequence is completely …

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K360121 K360121]: the LOVTap (blue light receptor) and it derivatives were not send from the registry

They were kindly provided by Dr. Chris E. French (University of Edinburgh).

[http://partsregistry.org/Part:BBa_K360051 K360051]: CcaS (green light receptor) is not yet available from the registry.

We are trying to build the green light response cascade from the Synechocystis sp. PCC6803 genomic DNA.