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Team:Freiburg/Project
From 2011.igem.org
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By eliminating routine use of expensive materials, this novel assay will utilize sustainable laboratory equipment and widespread His-Tag technology. | By eliminating routine use of expensive materials, this novel assay will utilize sustainable laboratory equipment and widespread His-Tag technology. | ||
| - | We propose an expression system induced by blue, green and red light, combined with subsequent temperature controlled autolysis of E. coli. | + | We propose an expression system induced by blue, green and red light, combined with subsequent temperature controlled autolysis of ''E. coli''. |
| - | Purification of the protein of interest will be accomplished by | + | Purification of the his-tagged protein of interest will be accomplished by |
an adaptor protein of our own design which binds the His-Tag on one side and the surface of serological pipettes on the other. Two subsequent pipetting steps for washing and purification of the cell lysate will quickly elute the product. | an adaptor protein of our own design which binds the His-Tag on one side and the surface of serological pipettes on the other. Two subsequent pipetting steps for washing and purification of the cell lysate will quickly elute the product. | ||
Our system will provide expression and purification of Polymerase and Ligase, but will be easily expandable to any His-Tagged protein needed by the modern molecular biologist. | Our system will provide expression and purification of Polymerase and Ligase, but will be easily expandable to any His-Tagged protein needed by the modern molecular biologist. | ||
{{:Team:Freiburg/Templates/footer}} | {{:Team:Freiburg/Templates/footer}} | ||
Revision as of 13:18, 14 July 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
