Team:Freiburg/Project

From 2011.igem.org

(Difference between revisions)
(Project overview)
(Project overview)
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We propose an expression system induced by blue, green and red light, combined with subsequent temperature controlled autolysis of ''E. coli''.  
We propose an expression system induced by blue, green and red light, combined with subsequent temperature controlled autolysis of ''E. coli''.  
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Purification of the his-tagged protein of interest will be accomplished by  
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The purification of the His-tagged protein of interest will be accomplished by  
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an adaptor protein of our own design which binds the His-Tag on one side and the surface of serological pipettes on the other. Two subsequent pipetting steps for washing and purification of the cell lysate will quickly elute the product.
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an adaptor protein of our own design which binds the His-tag on one side and the surface of serological pipettes on the other. Two subsequent pipetting steps for washing and purification of the cell lysate will quickly elute the product.
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Our system will provide expression and purification of Polymerase and Ligase, but will be easily expandable to any His-Tagged protein needed by the modern molecular biologist.
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Our system will provide expression and purification of Polymerase and Ligase, but will be easily expandable to any His-tagged protein needed by the modern molecular biologist.
== About ==
== About ==

Revision as of 16:59, 20 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!