Team:Freiburg/Notebook/8 June

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(Protein modelling and amplification of parts)
 
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==??.Labday==
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{{:Team:Freiburg/Templates/header}}
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==protein modelling==
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'''Investigators: Rüdiger'''<br/>
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<html>
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==Amplification of different parts==<br/> '''Investigators: Julia and Jacob'''
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<div id="notebook-page-header">
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<div id="notebook-back" width="100px" >
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/7_June">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 8 June </a>
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</div>
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<div id="notebook-next">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/9_June">Next entry</a>
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==<span style="color:grey;">Precipitator</span>==
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===Protein modelling with pymol===
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'''Investigators: Rüdiger'''
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<br/>
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<br/>
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==Amplification of different parts==
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'''Investigators: Julia and Jacob'''
antibiotics plates:<br/>
antibiotics plates:<br/>
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1oo mg/ml Ampizilin (100 microliter per plate)<br/>
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1oo mg/ml ampicillin (amp) (100 microliter per plate)<br/>
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1oo mikroliter H2O + 2 microliter Cm<br/>
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1oo mikroliter H2O + 2 microliter canamycin (cm)<br/>
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1oo mikroliter H2O + 40 microliter Tet<br/>
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1oo mikroliter H2O + 40 microliter tetracyclin(tet)<br/>
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1oo mikroliter H2O + 10 microliter Kan<br/>
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1oo mikroliter H2O + 10 microliter kanamycin (kn)<br/>
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{| border="1" align="center" style="text-align:center;"
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{| border="1" style="text-align:left;"
|'''part'''
|'''part'''
|'''location(p=plate)  '''
|'''location(p=plate)  '''
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|pSB1K3
|pSB1K3
|P1, 5A
|P1, 5A
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|kana
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|kan
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|
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|high copy BioBrick assemby plasmid
|-
|-
|pSB1A3
|pSB1A3
|P1, 1G
|P1, 1G
|amp
|amp
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|
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|high copy BioBrick assemby plasmid
|-
|-
|pSB1C3
|pSB1C3
|P1,3A
|P1,3A
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|chloramphenicol
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|cm
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|
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|high copy BioBrick assemby plasmid
|-
|-
|pSB1T3
|pSB1T3
|P1, 7A
|P1, 7A
|tet
|tet
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|
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|high copy BioBrick assemby plasmid
|-
|-
|J23104
|J23104
|P1, 18K
|P1, 18K
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|
-
|
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|promotor
|-
|-
|J23110
|J23110
|P1, 20 C
|P1, 20 C
|
|
-
|
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|promotor
|-
|-
|J23116
|J23116
|P1, 20M
|P1, 20M
|
|
-
|
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|promotor
|-
|-
|B0034
|B0034
|P1, 2M
|P1, 2M
|
|
-
|
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|RBS (strong)
|-
|-
|B0032
|B0032
|P1, 2 I
|P1, 2 I
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|
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|
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|RBS.3 (medium)
|-
|-
|B0031
|B0031
|P1, 2G
|P1, 2G
|
|
-
|
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|RBS.2 (weak)
|}
|}
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Transformation of parts in competent cells and plating them out on plates with specific antiobiotic resistance.
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{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:18, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!