Team:Freiburg/Notebook/3 September

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(Testdigestion of 2a-assembly)
 
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{{:Team:Freiburg/Templates/header}}
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/2_September">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 3 September </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/4_September">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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[[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]]
[[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]]
<br/>
<br/>
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the samples 46b= ho1 with a RBS (BBa_B0034)<br/>
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50b= RBS(BBa_B0034)+pcyA+terminator<br/>,
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the samples
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46b= ho1 with a RBS (BBa_B0034)<br/>
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50b= RBS(BBa_B0034)+pcyA+terminator<br/>
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64b= Promotor (BBa_J23110)+CcaS<br/>  
64b= Promotor (BBa_J23110)+CcaS<br/>  
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67b= romotor (BBa_J23110)+CcaR<br/>
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were send to GATC for sequencing
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67b= Promotor (BBa_J23110)+CcaR<br/>
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were send on [https://2011.igem.org/Team:Freiburg/Notebook/6_September 6.09] to GATC for sequencing
===3A-assembly:Digestion===
===3A-assembly:Digestion===
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'''InsertI:'''
'''InsertI:'''
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*64
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*64, CcaR
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*46  
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*46, ho1
with EcorI and SpeI
with EcorI and SpeI
'''InsertII:'''
'''InsertII:'''
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*67
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*67, CcaS
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*50
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*50, pcyA
with XbaI and PstI
with XbaI and PstI
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Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hour.
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For this assembly we used the constructs from the 2A-assembly made on [https://2011.igem.org/Team:Freiburg/Notebook/31_August 31.08]
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Incubation for 6 hours at 37°C. Vector pSB1C3 was already digested,<br/>  it was just treated with antarctic phosphatase for one hour.
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===3A-assembly:Ligation===
===3A-assembly:Ligation===

Latest revision as of 00:27, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!