Team:Freiburg/Notebook/3 September

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(3A-assembly:Digestion)
 
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{{:Team:Freiburg/Templates/header}}
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/2_September">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 3 September </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/4_September">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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'''Investigators: Julia'''
'''Investigators: Julia'''
<br/>
<br/>
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Continuing from  
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Continuing from 31 of Aug. [https://2011.igem.org/Team:Freiburg/Notebook/31_August]<br/>
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Before Miniprep of 20 differnt colonies.<br/>
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Before Miniprep of 20 different colonies.<br/>
Each minipreped DNA-sample (3μl)was digested with EcoRI and PstI.<br/>
Each minipreped DNA-sample (3μl)was digested with EcoRI and PstI.<br/>
<br/>
<br/>
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<br/>
<br/>
[[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]]
[[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]]
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<br/>
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the samples
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46b= ho1 with a RBS (BBa_B0034)<br/>
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50b= RBS(BBa_B0034)+pcyA+terminator<br/>
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64b= Promotor (BBa_J23110)+CcaS<br/>
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67b= Promotor (BBa_J23110)+CcaR<br/>
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were send on [https://2011.igem.org/Team:Freiburg/Notebook/6_September 6.09] to GATC for sequencing
===3A-assembly:Digestion===
===3A-assembly:Digestion===
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'''InsertI:'''
'''InsertI:'''
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*64
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*64, CcaR
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*46  
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*46, ho1
with EcorI and SpeI
with EcorI and SpeI
'''InsertII:'''
'''InsertII:'''
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*67
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*67, CcaS
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*50
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*50, pcyA
with XbaI and PstI
with XbaI and PstI
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Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hours.
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For this assembly we used the constructs from the 2A-assembly made on [https://2011.igem.org/Team:Freiburg/Notebook/31_August 31.08]
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Incubation for 6 hours at 37°C. Vector pSB1C3 was already digested,<br/>  it was just treated with antarctic phosphatase for one hour.
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===3A-assembly:Ligation===
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'''Investigators: Julia'''
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
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To do: Trafo
To do: Trafo
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===Transformation===
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with ♥C3 Lig. and Not C3 Lig
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'''Investigator: Sophie'''
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Latest revision as of 00:27, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!