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Team:Freiburg/Notebook/19 July
From 2011.igem.org
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'''Investigators: Sandra, Sophie''' | '''Investigators: Sandra, Sophie''' | ||
| - | Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor | + | Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor<br/> |
| - | + | <br/> | |
| - | LOVtap-Trp_up gaattcgcggccgcttctagtcacacaggaaagtactatgt | + | LOVtap-Trp_up gaattcgcggccgcttctagtcacacaggaaagtactatgt<br/> |
| - | LOVtap-Trp_dw tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg | + | LOVtap-Trp_dw tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg<br/> |
| - | TetR-TetO_up aaagaggagaaatactagatgtccagattag | + | TetR-TetO_up aaagaggagaaatactagatgtccagattag<br/> |
| - | TetR-TetO_dw* ctgcagcggccgctactag | + | TetR-TetO_dw* ctgcagcggccgctactag<br/> |
| - | *we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed. | + | *we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.<br/> |
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
Revision as of 14:12, 19 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Theoretical Gibson-Assembly
Investigators: Sandra, Sophie
Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor
LOVtap-Trp_up gaattcgcggccgcttctagtcacacaggaaagtactatgt
LOVtap-Trp_dw tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg
TetR-TetO_up aaagaggagaaatactagatgtccagattag
TetR-TetO_dw* ctgcagcggccgctactag
- we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
