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Team:Freiburg/Notebook/19 July
From 2011.igem.org
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==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
| - | === | + | ===Theoretical Gibson-Assembly=== |
| - | + | ||
| - | + | ||
| + | '''Investigators: Sandra, Sophie''' | ||
| + | Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor | ||
| + | LOVtap-Trp_up gaattcgcggccgcttctagtcacacaggaaagtactatgt | ||
| + | LOVtap-Trp_dw tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg | ||
| + | TetR-TetO_up aaagaggagaaatactagatgtccagattag | ||
| + | TetR-TetO_dw* ctgcagcggccgctactag | ||
| + | *we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed. | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
Revision as of 14:12, 19 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Theoretical Gibson-Assembly
Investigators: Sandra, Sophie
Primer Degin for Blue light sensor (lovTAP) + trp promotor and tetR Gen + tetO promotor
LOVtap-Trp_up gaattcgcggccgcttctagtcacacaggaaagtactatgt LOVtap-Trp_dw tacttttatctaatctggacatctagtatttctcctctttgtcgataccctttttacgtg TetR-TetO_up aaagaggagaaatactagatgtccagattag TetR-TetO_dw* ctgcagcggccgctactag
- we also have another primer for this one, but we are not sure about the length/melting temperature. Might be changed.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
