Team:Freiburg/Notebook/15 August
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
- | === | + | ===2A Assembly of the Lysis Cassette (S4+S15)=== |
- | '''Investigators: | + | '''Investigators:Theo''' |
+ | Since 3A assemly doesn't want to help us, we are going to try a 2A Assembly of S4 with S15.<br> | ||
+ | S4 is cut with SpeI and PstI.<br> | ||
+ | S15 is cut with XbaI and PstI, and is to be run on a gel, cut, isolated, ligated with S4 and finally transformed in competent cells.<br> | ||
+ | |||
+ | After the gel with S15 was run, I noticed there was no band to be seen and our instructor told me it was because I had not used a lot of DNA (500ng). | ||
+ | <br> | ||
+ | |||
+ | I am going to repeat this tomorrow. | ||
==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== |
Revision as of 22:42, 21 September 2011