Team:Freiburg/Notebook/15 August

• < home >
• < team >
  • → members
  • → attributions
  • → freiburg
• < project >
  • → description
  • → results
  • → modeling
  • → notebook
  • → sample data
• < human practices >
  • → oath
  • → ethics
  • → safety
• < gallery >
• < sponsors >
• < to the forums >

green light receptor

Order primers

Investigators: Jakob

• Order new primers for the quickchange of CcaS and CcaR
• I got the sequence from the iGEM-Team Uppsala THX again!

blue light receptor

Transformation

Investigators: Sophie

Transformation of ligated parts:

• LovTAP
• Not-Gate
• T3 vector

stored in incubator at 37°C.

red light receptor

PCR for Cph8

Investigators:Julia

after protocol from Uppsala. PCR-Mixture for one Reaction:

For a 50 µl reaction use

Primer used:
cph8 Prefix: TTCGAATTCGCGGCCGCTTCTAGATGGCCACCACCGTACAA
cph8 Suffix: CCGCTACTAGTATTATTACCCTTCTTTTGTCATGCCCT

PCR program:
Temp. Time,Nr of cycles
98° 5' 1x
98° 30 10x
65° 30
72° 1'15
98° 30 20x
72° 1'15
72° 7' 1x
4° ∞

Template: Voights plasmid (10x dilution)
Amplicon: 2235 bp

Elongation time Phusion 67,05 sec

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

Ligation

Investigators: Sophie
continue from experiment: Cloning (12.8.11)
Project name: inducible promoter for pbd
Vector: psb1T3
Inserts: IPTG-Promoter,Pbd-GFP
samples stored in Ruediger's box

Transformation

Investigators: Sophie
continue from experiment: Ligation
Project name: inducible promoter for pbd
samples stored in incubator

PCR

Investigators: Rüdiger

PCR of precipitator.

Primer:

• p44
• p45
• p46
• p48
• 51
• 52
• 53

Primer:

• p54
• p47