Team:Freiburg/Notebook/13 July

From 2011.igem.org

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===Testdigest===
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'''Investigators: Julia'''
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Testdigest of cph8
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*Enzyme 1: EcoRI
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*Enzyme 2: PstI

Revision as of 12:20, 15 July 2011

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:

samples DNA concentration 260/280 230
cph8a 33.7 1.79 1.74
cph8b 41.2 1.79 1.39
cph8c 24.1 1.87 1.47
cph8d 30 1.74 1.84
cph8e 34 1.81 1.86
cph8f 30 1.83 1.81


Testdigest

Investigators: Julia

Testdigest of cph8

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI