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From 2011.igem.org

Miniprep of Promotor-Ribosome-binding-site-constructs

Investigators: Sophie As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain The DNA-concentrations measured were (in ng/µL):

PR1a: 89,7 PR1b: 98,6

PR2a: 78,2 PR2b: 52,4

PR3a: 25,7 PR3b: 37,8

PR4a: 45,7 PR4b: 15,7

PR5a: 35,1 PR5b: 55,9

PR6a: 45,7 PR6b: 38,9

Test-digest

Investigators: Julia

14 samples:

• M30-M32, M 36-M38?????????????????
• S24-S29

Enzymes used:

• Enzyme 1: EcoRI
• Enzyme 2: PstI

Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.

Transformation

Investigators: Rüdiger

To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells.