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Team:Freiburg/Notebook/11 July
From 2011.igem.org
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PR6a: 45,7 | PR6a: 45,7 | ||
PR6b: 38,9 | PR6b: 38,9 | ||
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| + | ==Test-digest== | ||
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| + | '''Investigators: Julia''' | ||
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| + | 14 samples: | ||
| + | *M30-M32, M 36-M38 | ||
| + | *S24-S29 | ||
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| + | Enzymes used: | ||
| + | *Enzyme 1: EcoRI | ||
| + | *Enzyme 2: PstI | ||
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| + | <span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel</span> | ||
Revision as of 11:33, 14 July 2011
Miniprep of Promotor-Ribosome-binding-site-constructs
As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain The DNA-concentrations measured were (in ng/µL):
PR1a: 89,7 PR1b: 98,6
PR2a: 78,2 PR2b: 52,4
PR3a: 25,7 PR3b: 37,8
PR4a: 45,7 PR4b: 15,7
PR5a: 35,1 PR5b: 55,9
PR6a: 45,7 PR6b: 38,9
Test-digest
Investigators: Julia
14 samples:
- M30-M32, M 36-M38
- S24-S29
Enzymes used:
- Enzyme 1: EcoRI
- Enzyme 2: PstI
Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel
