Team:Freiburg/Notebook/11 July

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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 July </a>
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==Miniprep of Promotor-Ribosome-binding-site-constructs==
==Miniprep of Promotor-Ribosome-binding-site-constructs==

Latest revision as of 00:54, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

Miniprep of Promotor-Ribosome-binding-site-constructs

Investigators: Sophie

As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain The DNA-concentrations measured were (in ng/µL):

PR1a: 89,7 PR1b: 98,6

PR2a: 78,2 PR2b: 52,4

PR3a: 25,7 PR3b: 37,8

PR4a: 45,7 PR4b: 15,7

PR5a: 35,1 PR5b: 55,9

PR6a: 45,7 PR6b: 38,9

reg light receptor

Test-digest

Investigators: Julia

14 samples:

  • M30-M32, M 36-M38
  • S24-S29

Enzymes used:

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI


caption

Image of S24-S26: pcyA and S27-S29: cph8

Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.

Precipitator

Transformation

Investigators: Rüdiger To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/11_July"