Team:Freiburg/Notebook/11 July
From 2011.igem.org
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+ | {{:Team:Freiburg/Templates/header}} | ||
+ | <html> | ||
+ | <div id="notebook-page-header"> | ||
+ | <div id="notebook-back" width="100px" > | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/8_July">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 July </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/12_July">Next entry</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
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==Miniprep of Promotor-Ribosome-binding-site-constructs== | ==Miniprep of Promotor-Ribosome-binding-site-constructs== | ||
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PR6b: 38,9 | PR6b: 38,9 | ||
- | ==Test-digest== | + | ==<span style="color:red;">reg light receptor</span>== |
+ | ===Test-digest=== | ||
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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*Enzyme 1: EcoRI | *Enzyme 1: EcoRI | ||
*Enzyme 2: PstI | *Enzyme 2: PstI | ||
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+ | [[File:Gimp_11_07_2011.jpg|none|caption]] | ||
+ | Image of S24-S26: pcyA and S27-S29: cph8 | ||
<span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.</span> | <span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.</span> | ||
- | ==<span style="color:grey;"> | + | ==<span style="color:grey;">Precipitator</span>== |
- | + | ===Transformation=== | |
'''Investigators: Rüdiger''' | '''Investigators: Rüdiger''' | ||
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To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells. | To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells. | ||
+ | {{:Team:Freiburg/Templates/footer}} |
Latest revision as of 00:54, 22 September 2011