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Team:Freiburg/Notebook/11 July
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==Miniprep of Promotor-Ribosome-binding-site-constructs== | ==Miniprep of Promotor-Ribosome-binding-site-constructs== | ||
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| + | '''Investigators: Sophie''' | ||
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As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain | As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain | ||
The DNA-concentrations measured were (in ng/µL): | The DNA-concentrations measured were (in ng/µL): | ||
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PR6b: 38,9 | PR6b: 38,9 | ||
| - | + | ==<span style="color:red;">reg light receptor</span>== | |
| - | + | ===Test-digest=== | |
| - | ==Test-digest== | + | |
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
14 samples: | 14 samples: | ||
| - | *M30-M32, M 36-M38 | + | *M30-M32, M 36-M38 |
*S24-S29 | *S24-S29 | ||
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*Enzyme 2: PstI | *Enzyme 2: PstI | ||
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| + | [[File:Gimp_11_07_2011.jpg|none|caption]] | ||
| + | Image of S24-S26: pcyA and S27-S29: cph8 | ||
| - | + | <span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.</span> | |
| + | ==<span style="color:grey;">Precipitator</span>== | ||
| + | ===Transformation=== | ||
'''Investigators: Rüdiger''' | '''Investigators: Rüdiger''' | ||
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To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells. | To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells. | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 00:54, 22 September 2011
Contact 
This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
Miniprep of Promotor-Ribosome-binding-site-constructs
Investigators: Sophie
As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain The DNA-concentrations measured were (in ng/µL):
PR1a: 89,7 PR1b: 98,6
PR2a: 78,2 PR2b: 52,4
PR3a: 25,7 PR3b: 37,8
PR4a: 45,7 PR4b: 15,7
PR5a: 35,1 PR5b: 55,9
PR6a: 45,7 PR6b: 38,9
reg light receptor
Test-digest
Investigators: Julia
14 samples:
- M30-M32, M 36-M38
- S24-S29
Enzymes used:
- Enzyme 1: EcoRI
- Enzyme 2: PstI
Image of S24-S26: pcyA and S27-S29: cph8
Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.
Precipitator
Transformation
Investigators: Rüdiger To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells.
