Team:Freiburg/Notebook/11 July
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+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 July </a> | ||
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==Miniprep of Promotor-Ribosome-binding-site-constructs== | ==Miniprep of Promotor-Ribosome-binding-site-constructs== | ||
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+ | '''Investigators: Sophie''' | ||
+ | |||
As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain | As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain | ||
The DNA-concentrations measured were (in ng/µL): | The DNA-concentrations measured were (in ng/µL): | ||
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PR6b: 38,9 | PR6b: 38,9 | ||
- | + | ==<span style="color:red;">reg light receptor</span>== | |
- | + | ===Test-digest=== | |
- | ==Test-digest== | + | |
'''Investigators: Julia''' | '''Investigators: Julia''' | ||
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*Enzyme 2: PstI | *Enzyme 2: PstI | ||
- | <span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel</span> | + | |
+ | [[File:Gimp_11_07_2011.jpg|none|caption]] | ||
+ | Image of S24-S26: pcyA and S27-S29: cph8 | ||
+ | |||
+ | <span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.</span> | ||
+ | |||
+ | ==<span style="color:grey;">Precipitator</span>== | ||
+ | ===Transformation=== | ||
+ | '''Investigators: Rüdiger''' | ||
+ | To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells. | ||
+ | {{:Team:Freiburg/Templates/footer}} |
Latest revision as of 00:54, 22 September 2011