Team:Freiburg/Modelling

From 2011.igem.org

(Difference between revisions)
(The core structure)
(The core structure)
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|[[File:Freiburg11_Seq1.png|250px]]
|[[File:Freiburg11_Seq1.png|250px]]
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The aminoacid sequence of a bacterial ligase
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The aminoacid sequence of a bacterial  
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we chose for our design
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ligase we chose for our design (PDB:3CVR)
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especially not with one of the black plague. We also did not know what amino acid pattern on the LRR motif would cause the toxic effect. Probably, there would have been no harm in using this protein alone, even if we did clone and express it, but why should we do that when we have a huge choice freely available anyway. Finally, we settled on the bacterial ligase PDB:3CVR. A ligase would be definitively harmless, especially since we planned to restructure it entirely.
especially not with one of the black plague. We also did not know what amino acid pattern on the LRR motif would cause the toxic effect. Probably, there would have been no harm in using this protein alone, even if we did clone and express it, but why should we do that when we have a huge choice freely available anyway. Finally, we settled on the bacterial ligase PDB:3CVR. A ligase would be definitively harmless, especially since we planned to restructure it entirely.
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|[[File:Freiburg11Modelling2.png|750px]]
|[[File:Freiburg11Modelling2.png|750px]]
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Caption
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Consensus sequences derived from the repeating loops of the 3CVR ligase to work out the conserved aminoacids and shared chemical properties of the different positions in the loops.
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Revision as of 03:33, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!