Team:Freiburg/Description

From 2011.igem.org

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(The Concept)
(The Concept)
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===The Concept===
===The Concept===
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The idea behind our lysis cassette was to be able to achieve cell lysis by simply heating the bacteria to 42°C for a short period of time. This was accomplished by combining the biobricks BBa_K098995 (temperature sensitive promoter) and BBa_K124017 (Bacteriophage λ lysis genes). Sequencing of these parts (carried out by GATC Biotech GmbH) showed some fundamental inconsistencies leading to a lot of time spent on fixing them. <br>
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The idea behind our lysis cassette was to be able to achieve cell lysis by simply heating the bacteria to 42°C for a short period of time. This was accomplished by combining the biobricks [http://partsregistry.org/Part:BBa_K098995 BBa_K098995] (temperature sensitive promoter) and [http://partsregistry.org/Part:BBa_K124017 BBa_K124017] (Bacteriophage λ lysis genes). Sequencing of these parts (carried out by GATC Biotech GmbH) showed some fundamental inconsistencies leading to a lot of time spent on fixing them. <br>
The sequences were corrected and the new biobricks [http://partsregistry.org/Part:BBa_K608351 BBa_K608351] and [http://partsregistry.org/Part:BBa_K608352 BBa_K608352] were sent to the registry, although the novel temperature sensitive lysis cassette composite's sequence was verified some 18 hours before the Wiki-freeze, so that it could not be sent to the registry on time.<br>
The sequences were corrected and the new biobricks [http://partsregistry.org/Part:BBa_K608351 BBa_K608351] and [http://partsregistry.org/Part:BBa_K608352 BBa_K608352] were sent to the registry, although the novel temperature sensitive lysis cassette composite's sequence was verified some 18 hours before the Wiki-freeze, so that it could not be sent to the registry on time.<br>
Nevertheless some preliminary OD-measurement results did hint at the expected functionality of the newly developed composite part.<br>
Nevertheless some preliminary OD-measurement results did hint at the expected functionality of the newly developed composite part.<br>

Revision as of 19:56, 21 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!