Team:Freiburg/Description

From 2011.igem.org

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(Red light receptor)
(Bacterial artificial chromosome)
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We want to put most of our parts in a BAC so it can be used as a “Lab in a cell” system.<br/> This is very easy to handle and is a persistent transformation. Combined with our BAC vector we also have a transient plasmid with promoters for the expression of the His-tagged protein you want to purify. This protein can be cloned after these promoters and can be expressed with the influence of light (for example blue light). With this single cloning step, you can express your protein of interest. With the Bac vector all the other proteins are expressed dependent on the light exposure and heat.
We want to put most of our parts in a BAC so it can be used as a “Lab in a cell” system.<br/> This is very easy to handle and is a persistent transformation. Combined with our BAC vector we also have a transient plasmid with promoters for the expression of the His-tagged protein you want to purify. This protein can be cloned after these promoters and can be expressed with the influence of light (for example blue light). With this single cloning step, you can express your protein of interest. With the Bac vector all the other proteins are expressed dependent on the light exposure and heat.
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[[File:Freiburg2011_BAC.jpg]]
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Revision as of 02:52, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!