Team:Fatih Turkey/Project

From 2011.igem.org

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="http://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Fatih_Turkey_logo.png|200px|right|frame]]
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|align="center"|[[Team:Fatih_Turkey | Team Example]]
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!align="center"|[[Team:Fatih_Turkey|Home]]
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!align="center"|[[Team:Fatih_Turkey/Team|Team]]
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!align="center"|[http://igem.org/Team.cgi?year=2010&team_name=Fatih_Turkey Official Team Profile]
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!align="center"|[[Team:Fatih_Turkey/Project|Project]]
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!align="center"|[[Team:Fatih_Turkey/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Fatih_Turkey/Modeling|Modeling]]
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== '''The Rainbow Graveyard''' ==
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The story had began with a series of undefined infection events in humans which have been thought due to a gang of E.coli for decades. Only evidences at the scenes of the crimes are lipopolysaccharide (LPS) layer traces, thought to be used in all crimes of infections commited by the gram-negative gang. In our story, we will tell how detective B.subtilis and its collegues can catch the gang by synthesizing Limulus anti-LPS factor (LALF) on biofilm. So, LALF can detect the gang and inhibit its growth because of a high affinity binding for LPS. The police can use also LALF included biofilm onto surfaces to inhibit growth of other gram-negative individuals in the town to prevent their infections. Next, after each arresting of the E.coli gang by police, colour of the town has changed. The secret of the change is colourful feature of Reflectin protein synthesized by B.subtilis police squad and the gang. Reflectin is transfered into all individuals at ‘The Canvas Town’.
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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<a href="http://2011.igem.org/Team:Fatih_Turkey">Home</a>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Project</a>
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<ul>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li>
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</li>
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<li>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a>
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</li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Team">Team</a>
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<ul>
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                                                                                <li><a
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href="http://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li>
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<li>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
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<ul>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li>
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                                                                                <li><a
 +
href="http://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li>
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<div class="bar-title-whole">
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  <div class="bar-title">
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      <h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2>
 +
      <h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Overall Project</h5>
 +
    </div>
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  <div class="bar-title-sh-left"></div>
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<h1>The Rainbow Graveyard</h1>
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<center><img src="http://2011.igem.org/wiki/images/5/51/Growth_inh..png" width="400" /></center>
 +
<br>
 +
<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">E. Coli is one of the gram-negative bacteria and most of their types are harmless. However some of E.Coli types such as enterohaemorrhagic E.Coli (EHEC) can cause serious diseases.</p>
 +
<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">In our project we designed an innovative model to prevent gram-negative growth and infection. In our model, to prevent E.Coli growth, we modified non-infectious gram-positive bacteria B.Subtilis by transforming it with a construct, which was designed to produce limulus anti-LPS factor (LALF) together with a signal peptide. LALF is expected to bind and neutralize Lipopolysaccharide (LPS) found in gram-negative bacteria cell wall. On the other hand, to show E.Coli growth inhibition, we prepared another construct, which carries reflectin sequence with a signal peptide sequence and transferred into E.Coli. Reflectin protein produces color by reflecting light in different wavelenghts. This ability helps us to detect whether E.Coli is dead or alive...</p>
 +
 +
  <p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">We use two proteins which are anti-LPS (LALF) and Reflectin 1a. Anti-LPS protein can bind to LPS coat of gram negatives. With this function, the protein can be used as protection from septic shock. Also, anti LPS protein works as bacteriostatic.
 +
Reflectin 1a is a camouflage material and the protein is produced by many cephalopods (squid, cuttlefish, and octopus).<br>
 +
In this project, our first aim is to obtain an anti-gram negative bacterial surface with the help of anti-LPS protein. Our second aim is to sense the bacteriostatic effect with colorful reflectin protein. Also, we intend to apply our proteins on a surface as a coat.<br>
 +
We choose <a href="http://2011.igem.org/Team:Fatih_Turkey/Sporocide">Bacillus Subtilis</a> as the host of anti-LPS protein. Because, those bacteria are expected to produce biofilm containing the anti-LPS protein. This ability might help us to apply it on a surface.  </p>
-
== Results ==
+
<center>
 +
<img src="http://2011.igem.org/wiki/images/5/59/Overall1.png"/>
 +
<img src="http://2011.igem.org/wiki/images/5/55/Overall2.png.jpg"/>
 +
</center>
 +
 
 +
<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">To measure the inhibition effect properly, both of our bacteria are expected to synthesize reflectin. It is planned that anti-LPS exists in the biofilm will bind to LPS on the cell wall of Gram (-) bacteria, thus, Gram (-) bacteria will not grow and survive in our biofilm surface. Around the binding, reflectin thickness is going to decrease and change the color of the surface because of inhibition of growth of E.coli. So, if the color changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.
 +
 
 +
You can see for more information about LALF, Reflectin and Biofilm in the below links:
 +
</p>
 +
 
 +
<a style="float:left; text-align:center; margin-right:60px; margin-left:150px" href="http://2011.igem.org/Team:Fatih_Turkey/LALF">
 +
<h2>LALF (Anti-LPS)</h2>
 +
<img src="http://2011.igem.org/wiki/images/e/e7/Lalficon.png"/>
 +
</a>
 +
 
 +
<a style="float:left; text-align:center; margin-right:60px;" href="http://2011.igem.org/Team:Fatih_Turkey/Reflectin">
 +
<h2>Reflectin</h2>
 +
<img src="http://2011.igem.org/wiki/images/e/e1/Reflectinicon.png"/>
 +
</a>
 +
 
 +
<a style="float:left; text-align:center; margin-right:60px;" href="http://2011.igem.org/Team:Fatih_Turkey/Biofilm">
 +
<h2>Biofilm</h2>
 +
<img src="http://2011.igem.org/wiki/images/6/62/Biofilmicon.png"/>
 +
</a>
 +
 
 +
</pre>
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<h3 style="color:#BBB;">Sponsors</h3>
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<img height="100" src="http://2011.igem.org/wiki/images/7/72/Tubitak_-_Kopya.png"/>
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<a href="http://www.sentegen.com">
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Latest revision as of 20:59, 28 October 2011

deneme baslik

The Rainbow Graveyard


E. Coli is one of the gram-negative bacteria and most of their types are harmless. However some of E.Coli types such as enterohaemorrhagic E.Coli (EHEC) can cause serious diseases.

In our project we designed an innovative model to prevent gram-negative growth and infection. In our model, to prevent E.Coli growth, we modified non-infectious gram-positive bacteria B.Subtilis by transforming it with a construct, which was designed to produce limulus anti-LPS factor (LALF) together with a signal peptide. LALF is expected to bind and neutralize Lipopolysaccharide (LPS) found in gram-negative bacteria cell wall. On the other hand, to show E.Coli growth inhibition, we prepared another construct, which carries reflectin sequence with a signal peptide sequence and transferred into E.Coli. Reflectin protein produces color by reflecting light in different wavelenghts. This ability helps us to detect whether E.Coli is dead or alive...

We use two proteins which are anti-LPS (LALF) and Reflectin 1a. Anti-LPS protein can bind to LPS coat of gram negatives. With this function, the protein can be used as protection from septic shock. Also, anti LPS protein works as bacteriostatic. Reflectin 1a is a camouflage material and the protein is produced by many cephalopods (squid, cuttlefish, and octopus).
In this project, our first aim is to obtain an anti-gram negative bacterial surface with the help of anti-LPS protein. Our second aim is to sense the bacteriostatic effect with colorful reflectin protein. Also, we intend to apply our proteins on a surface as a coat.
We choose Bacillus Subtilis as the host of anti-LPS protein. Because, those bacteria are expected to produce biofilm containing the anti-LPS protein. This ability might help us to apply it on a surface.

To measure the inhibition effect properly, both of our bacteria are expected to synthesize reflectin. It is planned that anti-LPS exists in the biofilm will bind to LPS on the cell wall of Gram (-) bacteria, thus, Gram (-) bacteria will not grow and survive in our biofilm surface. Around the binding, reflectin thickness is going to decrease and change the color of the surface because of inhibition of growth of E.coli. So, if the color changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor. You can see for more information about LALF, Reflectin and Biofilm in the below links:

LALF (Anti-LPS)

Reflectin

Biofilm