Team:Fatih Turkey/Parts
From 2011.igem.org
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+ | <!-- Logo --> | ||
+ | <div> | ||
+ | <div class="logo"> | ||
+ | <a href="https://2011.igem.org/Team:Fatih_Turkey"> | ||
+ | <img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"> | ||
+ | </a> | ||
+ | </div> | ||
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+ | <ul id="menu-main" class="menu"> | ||
+ | <li> | ||
+ | <a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a> | ||
+ | </li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a> | ||
+ | <ul> | ||
+ | <li><a | ||
+ | href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li> | ||
+ | <li><a | ||
+ | href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li> | ||
+ | <li><a | ||
+ | href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li> | ||
+ | <li><a | ||
+ | href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- End Header --> | ||
+ | <div class="bar-title-whole"> | ||
+ | <div class="bar-title"> | ||
+ | <h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Biobricks</h2> | ||
+ | <h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Parts</h5> | ||
+ | </div> | ||
+ | <div class="bar-title-sh-left"></div> | ||
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+ | <div id="page"> | ||
+ | <div class="page-wrapper"> | ||
+ | |||
+ | <style> | ||
+ | table{background:transparent !important;} | ||
+ | </style> <!-- post container --> | ||
+ | <div> | ||
+ | <div class="meta-left-full"> | ||
+ | |||
+ | <TABLE width='865px'><TR><TD width='50%'><B>Favorite Fatih_Turkey 2011 iGEM Team Parts</B><TD width='35%'><TD width='15%' ALIGN='RIGHT'></TABLE> | ||
+ | <TABLE border="1" class='pgrouptable addborder' style='' cellpadding='0' cellspacing='0'> | ||
+ | <TR> | ||
+ | <TH style='width: 7em;'>Name</TH> | ||
+ | <TH colspan=''>Type</th> | ||
+ | <TH colspan=''>Description</th> | ||
+ | <TH colspan=''>Designer</th> | ||
+ | <TH colspan=''>Length</th> | ||
+ | </tr> | ||
+ | |||
+ | <!-- ROW --> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21720" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541515'>BBa_K541515</A></TD><TD>Device</TD><TD>Limulus anti-LPS factor (LALF) for Bacillus subtilis</TD><TD width='100px'>Cihan TAŞTAN</TD><TD ALIGN='right'>537</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21732" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541545'>BBa_K541545</A></TD><TD>Device</TD><TD>Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>767</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="22805" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541800'>BBa_K541800</A></TD><TD>Plasmid</TD><TD>B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>6285</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="22806" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541915'>BBa_K541915</A></TD><TD>Plasmid</TD><TD>Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>6109</TD></FORM></TABLE></FORM><BR><BR> | ||
+ | |||
+ | |||
+ | <!-- Show=Undef --> | ||
+ | <TABLE width='865px'><TR><TD width='50%'><B>Fatih_Turkey 2011 iGEM Team Parts Sandbox</B><TD width='35%'><TD width='15%' ALIGN='RIGHT'></TABLE> | ||
+ | <TABLE border="1" class='pgrouptable addborder' style='' cellpadding='0' cellspacing='0'><TR><TH style='width: 7em;'>Name</TH><TH colspan=''>Type<TH colspan=''>Description<TH colspan=''>Designer<TH colspan=''>Length | ||
+ | |||
+ | <!-- ROW --> | ||
+ | |||
+ | <!-- ROW --> | ||
+ | |||
+ | <!-- ROW --> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21878" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541003'>BBa_K541003</A></TD><TD>Device</TD><TD>Reflectin 1A with promoter and RBS</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1081</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21738" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541024'>BBa_K541024</A></TD><TD>Composite</TD><TD>Reflectin 1A (SacB) and LALF (SacB) protein for B.Subtilis</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1523</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21739" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541025'>BBa_K541025</A></TD><TD>Composite</TD><TD>Reflectin 1A (LipA) and LALF (SacB) protein for B.subtilis</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1631</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21740" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541026'>BBa_K541026</A></TD><TD>Composite</TD><TD>Reflectin 1A (SacB) and LALF (LipA) protein for B.subtilis</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1529</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21746" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541027'>BBa_K541027</A></TD><TD>Composite</TD><TD>Reflectin 1A (LipA) and LALF (LipA) protein for B.subtilis</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1637</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21747" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541028'>BBa_K541028</A></TD><TD>Composite</TD><TD>Reflectin 1A (TAT-Constitutive) and LALF (TAT-IPTG inducable) protein for E.coli</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1787</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21748" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541029'>BBa_K541029</A></TD><TD>Composite</TD><TD>Reflectin 1A (TAT-IPTG inducable) and LALF (TAT-IPTG inducable) protein for E.coli</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>2085</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21726" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541501'>BBa_K541501</A></TD><TD>Regulatory</TD><TD>Promoter Pveg, RBS spoVG and SacB signal peptide for B. subtilis</TD><TD width='100px'>Cihan TAŞTAN</TD><TD ALIGN='right'>219</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21731" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541502'>BBa_K541502</A></TD><TD>Regulatory</TD><TD>Promoter Pveg, RBS spoVG and LipA signal peptide for B. subtilis</TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>225</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21728" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541503'>BBa_K541503</A></TD><TD>Regulatory</TD><TD>Constitutive Strong promoter, Strong RBS and TAT signal peptide</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>151</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21730" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541504'>BBa_K541504</A></TD><TD>Regulatory</TD><TD>IPTG inducible PlacI, RBS and TAT signal peptide</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>449</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21729" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541505'>BBa_K541505</A></TD><TD>Generator</TD><TD>limulus anti-LPS factor (LALF)</TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>312</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21727" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541506'>BBa_K541506</A></TD><TD>Generator</TD><TD>Reflectin1A from Cephalopod</TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>855</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21734" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541516'>BBa_K541516</A></TD><TD>Device</TD><TD>Reflectin1A for B.subtiis with SacB signal sequence</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>978</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21733" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541525'>BBa_K541525</A></TD><TD>Device</TD><TD>LALF for Bacillus subtilis with LipA signal peptide</TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>543</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21735" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541526'>BBa_K541526</A></TD><TD>Device</TD><TD>reflectin for b.subtilis with LipA signal peptide</TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>1086</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21736" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541536'>BBa_K541536</A></TD><TD>Device</TD><TD>Reflectin 1A for E.coli with TAT signal sequence (Constitutive)</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>1012</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="21737" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541546'>BBa_K541546</A></TD><TD>Device</TD><TD>IPTG inducible PlacI, RBS,TAT signal peptide and reflectin</TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>1310</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="22803" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541596'>BBa_K541596</A></TD><TD>Device</TD><TD>Reflectin gene with J04500 promoter </TD><TD width='100px'>Fazilet Guler</TD><TD ALIGN='right'>1081</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="22832" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541815'>BBa_K541815</A></TD><TD>Plasmid</TD><TD>B.subtilis and E.coli Episomal Shuttle Vector with LALF and SacB signal peptide</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>5753</TD></FORM> | ||
+ | |||
+ | <form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Fatih_Turkey" enctype="multipart/form-data"> | ||
+ | <input type="hidden" name="Editing" value="Editing" /><input type="hidden" name="part" value="22830" /><input type="hidden" name="pgroup" value="iGEM2011" /><input type="hidden" name="group" value="Fatih_Turkey" /><TR><TD><A class='noul_link' href='http://partsregistry.org/wiki/index.php?title=Part:BBa_K541925'>BBa_K541925</A></TD><TD>Plasmid</TD><TD>Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and LipA signal peptide</TD><TD width='100px'>Mustafa Elitok</TD><TD ALIGN='right'>6115</TD></FORM></TABLE></FORM><BR><!-- end content --> | ||
+ | |||
+ | |||
+ | <p><strong><img src="https://static.igem.org/mediawiki/2011/b/bf/K541501.png" width="400" height="100" /></strong></p> | ||
+ | <p><strong>Promoter Pveg, RBS spoVG and SacB signal peptide for B. Subtilis; (BBa_K541501)</strong><br /> | ||
+ | This is the subpart of SacB signal peptide which is commonly used in our BioBricks. Signal peptide is used for the secretion of our protein outside of the cell. Promoter and RBS are compatible with B.subtilis.<strong></strong></p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/f/f7/K541502.png" width="400" height="100" /><br /> | ||
+ | <strong>Promoter Pveg, RBS spoVG and LipA signal peptide for B. Subtilis; (BBa_K541502)</strong><br /> | ||
+ | Subpart of LipA signal peptide is designed alternatively for BBa_K541501. Promoter and RBS are compatible with B.subtilis.<strong></strong></p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/3/37/K541503.png" width="400" height="100" /><br /> | ||
+ | <strong>Constitutive Strong promoter, Strong RBS and TAT signal peptide; (BBa_K541503)</strong><br /> | ||
+ | The aim of design of this part is same with BBa_K541501 and BBa_K541502. This part is compatible with only E.coli because of its promoter and RBS. Tat signal peptide is inserted in this gene.<strong></strong></p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/d/d1/K541504.png" width="400" height="100" /><br /> | ||
+ | <strong>IPTG inducible PlacI, RBS and TAT signal peptide; (BBa_K541504)</strong><br /> | ||
+ | It is same with BBa_K541503. Differently, we used IPTG inducible PlacI promoter with E.coi compatible RBS.<strong></strong></p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/9/9f/K541505.png" width="400" height="100" /><br /> | ||
+ | <strong>Limulus anti-LPS factor (LALF); (BBa_K541505)</strong><br /> | ||
+ | LALF subpart that we inserted in our BioBricks which are related to LALF. It has been synthesized in vitro in order to prevent horseshoe crab death, sea creature that hosts LALF. For more information; visit our<a href="https://2011.igem.org/Team:Fatih_Turkey/LALF"> LALF page.</a></p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/a/a1/K541506.png" width="400" height="100" /><br /> | ||
+ | <strong>Reflectin1A from Cephalopod</strong><strong>; (BBa_K541506)</strong><br /> | ||
+ | Reflectin is aimed to use as a biosensor in our project. This is the subpart of parts for reflectin. </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/f/fe/K541515.png" width="900" height="139" /><br /> | ||
+ | <strong>Limulus anti-LPS factor (LALF) for Bacillus subtilis</strong>; <strong>BBa_K541515</strong><br /> | ||
+ | We designed this part in order to stop E.coli growth via B.subtilis. Constitutive promoter veg (BBa_K143012) and RBS spoVG (BBa_K143021) are inserted to be able to use the device in B.subtilis. With help of signal peptide, the protein will be synthesized outside of the cell easily. SacB signal peptide is one of responsible systems in Sec-SRP pathway in B.subtilis. Secreting outside allows us to test whether we could have a solution that does not include any bacterium; thus solution could be used as anti-septic. We tested this effect by comparing the exposure of liquid culture and supernatant of B.subtilis on E.coli.</p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><strong>Reflectin gene with J04500 promoter; </strong><strong>BBa_K541596</strong> <br /> | ||
+ | Reflectin 1A, a protein that can be found in Cephalopod iridocyte cells, has the ability to reflect the white light by changing its wavelength depending on the thickness of the protein structure. Because of this, the surface or the cell covered with this protein is seen colorful. This ability, we believe, will allow us to test efficiency of inhibition by LALF. We planned that in the case of prevention of growth, reflectin thickness will be different between experimental and control groups; thus shows that LALF works. Although we want to secrete this protein outside of the cell in order to have a better thick structure output, we also wondered what would happen if protein remains in the cell. That is the reason that we designed this part which does not include signal peptide sequence. In addition, IPTG inducible PlacI promoter and B0034 RBS are included in the part.</p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/a/a4/K541545.png" width="834" height="139" /><br /> | ||
+ | <strong>Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible); </strong><strong>BBa_K541545</strong> <br /> | ||
+ | Besides of inhibiting gram negative growth via B.subtilis, we also wondered what if E.coli synthesizes LALF to stop its own growth. IPTG inducible promoter PlacI and RBS B0034 are selected for this reason in the part, in order to test its effectiveness properly. We added Tat signal peptide sequence to allow LALF to work efficiently. Tat signal system is one of major secretion pathways that exist in gram negatives.</p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2011/f/f4/K541800.png" width="300" height="150" /><br /> | ||
+ | <strong>B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli; </strong><strong>BBa_K541800</strong> <br /> | ||
+ | This episomal vector has within its BioBrick region a constitutively expressed RFP gene in E.coli. It uses LacI promoter, B0034, mRFP1 (E0010) and B0015.</p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p><br /> | ||
+ | <strong>Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide; BBa_K541915</strong> <br /> | ||
+ | Same gene components are included with BBa_K541515. Only difference here is the plasmid backbone we use. (BBa_I147123) This part is the major sequence that we use in our LALF experiments. </p> | ||
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Latest revision as of 23:52, 28 October 2011
2011 © Fatih Medical School