Team:Fatih Turkey/Notebook13

From 2011.igem.org

Revision as of 02:57, 29 October 2011 by Fazilet (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

deneme baslik

Week:

      We made like MİC(minimum inhibitory concentration) experiment which is using been for antibiotic because of finding minimum concentration of LALF  that get inhibition of growing e.coli.But we didn’t added  sequence which help us in purifying of protein like His-tag  which we were synthesising LALF .So we used supernatant of B.subtilis which produced LALF instead of pure LALF protein.
23.10.2011
We used culture of  e. coli whose OD is 0,4 with GFP.50 ul culture of e.coli and 3-50 ul supernatant of  B.subtilis with LALF and without LALF were  put into all wells.Lb with Chl  was added by completing total volume to 100 ul.
24.10.2011
We used culture of e.coli whose OD is 0,4 with GFP .25 ul culture of e.coli  and 75 ul supernatant of B.subtilis with LALF and without LALF were  put into all wells.Lb with Chl  was added by completing total volume to 100 ul.
25.10.2011
We used culture of e.coli whose OD is 0,1 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were  put into all wells.Lb with Chl  was added by completing total volume to 100 ul.
26.10.2011
We used culture of e.coli whose OD is 0,2 with GFP .25 ul culture of e.coli  and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells. Lb with Chl  was added by completing total volume to 100 ul.
27.10.2011
The experiment which was made for observing to supernatant of B.subtilis with LALF effect on liquid culture of e.coli.
6 ml LB with chl ,2ul e.coli which was growthed overnight ;10,100,1000,2000 ul  B.subtilis supernatant with LALF and without LALF was added.
B.subtilis supernatant with  LALF and  without LALF was completed to 2 ml with Lb has chl.
Measure of OD was made at 0-2-4-6-8 hours in 600 and 405 nm.
29.10.2011 E.coli with RFP was used.
1. We used 50ul e.coli culture whose OD came 0,1 with RFP and 50 ul LALF.
2. We used   25ul e.coli culture whose OD came 0,1 with RFP and 75ul LALF.
3.We used  90ul e.coli culture whose OD came 0,1 with RFP and 10ul LALF.

4. We used 25ul e.coli culture whose OD came 0,2 with RFP and 75 ul LALF.
5. We used 10ul e.coli culture whose OD came 0,2 with RFP and 90 ul LALF.
6. We used 25ul e.coli culture whose OD came 0,3 with RFP and 75ul LALF.
7. We used  10ul e.coli culture whose OD came 0,3 with RFP and 90ul LALF.
8. We used 10ul e.coli culture whose OD came 0,4 with RFP and 90ul LALF.