Team:Fatih Turkey/Notebook

From 2011.igem.org

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          14.07.11
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<h4>14.07.11</h4>
-
PROBLEMS AND SOLUTIONS
+
-
# Prepare to competent materials;
+
PROBLEMS AND SOLUTIONS<br><br>
-
*We prepared the competent materials for tomorrow
+
-
(tomorrow we will prepare the competents of b. subtilis and it will be 5th time:))
+
-
*we prepared our b subtilis plates for using tomorrow
+
-
*we have two plates for competent, one of them is wild type and other stain is ATCC6633
+
-
*we named them not to confuse, the ATCC663 strain's new name is Ahmet, wild type bacteria's new name is Mahmut :)
+
-
those names are turkish male names
+
-
# Transformation;
+
# Prepare to competent materials;<br>
-
*we couldn't get the expected results at transformation of b. subtilis in this week today we will try to make the most succesful transformation ever :)
+
-
The Problems and The Solutions:
+
-
-We decided to some changeings at our procedure of competent
+
-
*incubation time will be a hour, firstly we will add the DNA to b. subtilis pellet without medium B then we will shake the mix for 30 min. and we  will add medium B on this mix than we will shake new mix again for 30 min.
+
-
*amount of DNA will be increased
+
-
SITUATION
+
*We prepared the competent materials for tomorrow<br>
-
Transformation; we prepared 4 plates for transformation
+
 
-
1. plate : b. subtilis pellet + 6 microliter plasmid ---> incubation/shaker30 min.--> adding 100 microliter medium B --->incubation/shaker30min. --->spreading 5ug/ml Chm plate
+
(tomorrow we will prepare the competents of b. subtilis and it will be 5th time:))<br>
-
2. plate: b subtilis pellet + 10 microliter plasmid ---> incubation/shaker30 min.--> adding 100 microliter medium B --->incubation/shaker30min.----> spreading 5ug/ml  Chm plate
+
 
-
3. plate: b subtilis pellet + adding 100 microliter medium B ---> incubation/sahker 30 min.--->adding 10 microliter plasmid ---> incubation/shaker30min.----> spreading 5ug/ml Chm plate
+
*we prepared our b subtilis plates for using tomorrow<br>
-
4.plate: b subtilis pellet + adding 100 microliter medium B ---> incubation/sahker 30 min.--->adding 6 microliter plasmid ---> incubation/shaker30min.----> spreading 5ug/ml Chm plate       </div>
+
 
 +
*we have two plates for competent, one of them is wild type and other stain is ATCC6633<br>
 +
 
 +
*we named them not to confuse, the ATCC663 strain's new name is Ahmet, wild type bacteria's new name is Mahmut :)<br>those names are turkish male names<br><br>
 +
 
 +
# Transformation;<br>
 +
 
 +
*we couldn't get the expected results at transformation of b. subtilis in this week today we will try to make the most succesful transformation ever :)<br>
 +
 
 +
The Problems and The Solutions:<br>
 +
 
 +
-We decided to some changeings at our procedure of competent<br>
 +
 
 +
*incubation time will be a hour, firstly we will add the DNA to b. subtilis pellet without medium B then we will shake the mix for 30 min. and we  will add medium B on this mix than we will shake new mix again for 30 min.<br>
 +
 
 +
*amount of DNA will be increased<br><br>
 +
 
 +
SITUATION<br>
 +
 
 +
Transformation; we prepared 4 plates for transformation<br>
 +
 
 +
1. plate : b. subtilis pellet + 6 microliter plasmid ---&gt; incubation/shaker30 min.--&gt; adding 100 microliter medium B ---&gt;incubation/shaker30min. ---&gt;spreading 5ug/ml Chm plate<br>
 +
 
 +
2. plate: b subtilis pellet + 10 microliter plasmid ---&gt; incubation/shaker30 min.--&gt; adding 100 microliter medium B ---&gt;incubation/shaker30min.----&gt; spreading 5ug/ml  Chm plate<br>
 +
 
 +
3. plate: b subtilis pellet + adding 100 microliter medium B ---&gt; incubation/sahker 30 min.---&gt;adding 10 microliter plasmid ---&gt; incubation/shaker30min.----&gt; spreading 5ug/ml Chm plate<br>
 +
 
 +
4.plate: b subtilis pellet + adding 100 microliter medium B ---&gt; incubation/sahker 30 min.---&gt;adding 6 microliter plasmid ---&gt; incubation/shaker30min.----&gt; spreading 5ug/ml Chm plate<br>
 +
         
 +
    </div>
     </div>
     </div>

Revision as of 10:12, 28 October 2011

deneme baslik

Week:

14.07.11

PROBLEMS AND SOLUTIONS

# Prepare to competent materials;
*We prepared the competent materials for tomorrow
(tomorrow we will prepare the competents of b. subtilis and it will be 5th time:))
*we prepared our b subtilis plates for using tomorrow
*we have two plates for competent, one of them is wild type and other stain is ATCC6633
*we named them not to confuse, the ATCC663 strain's new name is Ahmet, wild type bacteria's new name is Mahmut :)
those names are turkish male names

# Transformation;
*we couldn't get the expected results at transformation of b. subtilis in this week today we will try to make the most succesful transformation ever :)
The Problems and The Solutions:
-We decided to some changeings at our procedure of competent
*incubation time will be a hour, firstly we will add the DNA to b. subtilis pellet without medium B then we will shake the mix for 30 min. and we  will add medium B on this mix than we will shake new mix again for 30 min.
*amount of DNA will be increased

SITUATION
Transformation; we prepared 4 plates for transformation
1. plate : b. subtilis pellet + 6 microliter plasmid ---> incubation/shaker30 min.--> adding 100 microliter medium B --->incubation/shaker30min. --->spreading 5ug/ml Chm plate
2. plate: b subtilis pellet + 10 microliter plasmid ---> incubation/shaker30 min.--> adding 100 microliter medium B --->incubation/shaker30min.----> spreading 5ug/ml  Chm plate
3. plate: b subtilis pellet + adding 100 microliter medium B ---> incubation/sahker 30 min.--->adding 10 microliter plasmid ---> incubation/shaker30min.----> spreading 5ug/ml Chm plate
4.plate: b subtilis pellet + adding 100 microliter medium B ---> incubation/sahker 30 min.--->adding 6 microliter plasmid ---> incubation/shaker30min.----> spreading 5ug/ml Chm plate