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Team:Fatih Turkey/Experiments4
From 2011.igem.org
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<td width="600" height="223" valign="top"><p><strong>Inhibition in Supernatant Assay</strong><br /> | <td width="600" height="223" valign="top"><p><strong>Inhibition in Supernatant Assay</strong><br /> | ||
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In this experiment, we wanted to investigate the effects of supernatant includes LALF protein to E.coli. Therefore, we put 8 ul LB broth into falcons. We added them E.coli with RFP and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for control. We added 1ul, 10ul and 100ul supernatant with LALF and without LALF.<br /> | In this experiment, we wanted to investigate the effects of supernatant includes LALF protein to E.coli. Therefore, we put 8 ul LB broth into falcons. We added them E.coli with RFP and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for control. We added 1ul, 10ul and 100ul supernatant with LALF and without LALF.<br /> | ||
We diluated them 7 times after 2 – 4 – 8 – 12 hours. Then, we spread 10 ul into plates which has half volume of spread amount chloramphenicol. We centrifuged the liquid cultures which incubated for 12 hours at 37⁰C at 7000 rpm for 5 minutes because of preparing supernatant with LALF and without LALF. All supernatants were passed through the 0,2 filter in order to decontaminate from bacteria and their disposals.</p></td> | We diluated them 7 times after 2 – 4 – 8 – 12 hours. Then, we spread 10 ul into plates which has half volume of spread amount chloramphenicol. We centrifuged the liquid cultures which incubated for 12 hours at 37⁰C at 7000 rpm for 5 minutes because of preparing supernatant with LALF and without LALF. All supernatants were passed through the 0,2 filter in order to decontaminate from bacteria and their disposals.</p></td> | ||
Latest revision as of 20:04, 28 October 2011
Inhibition in Supernatant Assay
In this experiment, we wanted to investigate the effects of supernatant includes LALF protein to E.coli. Therefore, we put 8 ul LB broth into falcons. We added them E.coli with RFP and incubated for 12 hours in 37⁰C .We put pure E.coli into only one falcon for control. We added 1ul, 10ul and 100ul supernatant with LALF and without LALF. |
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