Team:Fatih Turkey/Experiments

From 2011.igem.org

(Difference between revisions)
(Created page with "experiments")
Line 1: Line 1:
-
experiments
+
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
 +
<title>deneme baslik</title>
 +
<style>
 +
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{
 +
  display:none;
 +
}
 +
#menubar{
 +
background-color: transparent;
 +
font-size: 85%;
 +
top: 10px;
 +
position: absolute;
 +
margin-top: -12px;
 +
}
 +
 
 +
div#content{
 +
padding: 0 !important;
 +
background: transparent;
 +
border: none !important;
 +
width: auto;
 +
}
 +
 
 +
#menubar li a {
 +
color: #FFFFFF;
 +
background-color: transparent;
 +
}
 +
#globalWrapper{
 +
margin-top:5px;
 +
}
 +
#top-section{
 +
height: 20px !important;
 +
border: none !important;
 +
}
 +
body {
 +
font-size: 12px;
 +
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;
 +
color: #444;
 +
background: #6b6b6b url('http://igem.org/wiki/images/a/ad/Bg-igem.png');
 +
}
 +
body,p,pre,div,span{
 +
line-height: 160%;
 +
color: #333;
 +
}
 +
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,
 +
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td
 +
{
 +
margin:0;
 +
padding:0;
 +
border:0;
 +
font-weight:inherit;
 +
font-style:inherit;
 +
font-family:inherit;
 +
vertical-align:baseline;
 +
}
 +
 
 +
em{
 +
font-style:italic;
 +
}
 +
table{
 +
margin-top: 15px;
 +
margin-bottom: 15px;
 +
}
 +
hr{
 +
border: 0;
 +
color: #eee;
 +
background-color: #eee;
 +
height: 1px;
 +
margin-bottom: 20px;
 +
}
 +
img.centered {
 +
display: block;
 +
margin-left: auto;
 +
margin-right: auto;
 +
}
 +
img.alignnone {
 +
margin: 0px 0px 5px 5px;
 +
display: inline;
 +
}
 +
img.alignright {
 +
margin: 0px 0px 5px 10px;
 +
display: inline;
 +
}
 +
 
 +
img.alignleft {
 +
margin: 0px 10px 5px 0px;
 +
display: inline;
 +
}
 +
.alignright {
 +
float: right;
 +
}
 +
 
 +
.alignleft {
 +
float: left;
 +
}
 +
.aligncenter{
 +
margin-left: auto;
 +
margin-right: auto;
 +
}
 +
h1,h2,h3,h4,h5,h6{
 +
  margin-top: 15px;
 +
  margin-bottom: 15px ;
 +
  color: #333;
 +
  line-height: 1.5em;
 +
}
 +
h1 {
 +
font-size: 30px;
 +
}
 +
h2 {
 +
font-size: 25px;
 +
}
 +
h3 {
 +
font-size: 20px;
 +
}
 +
h4 {
 +
font-size: 18px;
 +
}
 +
h5 {
 +
font-size: 16px;
 +
}
 +
 
 +
h6 {
 +
font-size: 15px;
 +
}
 +
a{
 +
text-decoration: none;
 +
}
 +
a{
 +
color: #2f86c4;
 +
}
 +
a:hover{
 +
color: #4172ab;
 +
}
 +
#header .logo{
 +
margin-top: 41px;
 +
margin-left: 40px;
 +
}
 +
#footer{
 +
background: #707070;
 +
border-left: 1px #888 solid;
 +
border-right: 1px #888 solid;
 +
width: 988px;
 +
margin: 0px auto 0px;
 +
overflow: hidden;
 +
}
 +
#footer div{
 +
color: #bbb;
 +
}
 +
#footer a{
 +
color: #c4c5cc;
 +
text-decoration: none;
 +
}
 +
#footer a:hover{
 +
color: #666;
 +
}
 +
 
 +
.footer-sh-wrapper {
 +
    height: 22px;
 +
    margin: auto;
 +
    position: relative;
 +
    width: 990px;
 +
}
 +
.footer-sh-left {
 +
    background: url("http://2011.igem.org/wiki/images/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;
 +
    height: 112px;
 +
    left: 0;
 +
    position: absolute;
 +
    top: -90px;
 +
    width: 10px;
 +
}
 +
.footer-sh {
 +
    background: url("http://2011.igem.org/wiki/images/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;
 +
    bottom: 0;
 +
    height: 22px;
 +
    left: 10px;
 +
    position: absolute;
 +
    width: 970px;
 +
}
 +
.footer-sh-right {
 +
    background: url("http://2011.igem.org/wiki/images/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;
 +
    height: 112px;
 +
    position: absolute;
 +
    right: 0;
 +
    top: -90px;
 +
    width: 10px;
 +
}
 +
 
 +
.copyright-open{
 +
width: 988px;
 +
height: 0px;
 +
margin:auto;
 +
display: block-inline;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
color: #333333;
 +
}
 +
#header-whole{
 +
 
 +
position: relative;
 +
z-index: 200;
 +
width: 980px;
 +
margin: 0 auto;
 +
}
 +
#header{
 +
height: 100px;
 +
clear: both;
 +
background: #f9f9f9;
 +
margin: 0 auto;
 +
    width: 970px;
 +
}
 +
#header .logo{
 +
float: left;
 +
margin-top: 15px;
 +
margin-left: 20px;
 +
}
 +
 
 +
#container{
 +
width: 970px;
 +
margin: 0px auto 0px;
 +
background: none repeat scroll 0 0 #F9F9F9;
 +
}
 +
.copyright-open{
 +
border-top: 1px #a8a8a8 solid;
 +
border-bottom: 1px #888 solid;
 +
border-left: 1px #888 solid;
 +
border-right: 1px #888 solid;
 +
}
 +
.nav-wrapper{
 +
margin: 0px 90px 0px 0px;
 +
float:right;
 +
}
 +
.ddsmoothmenu ul{
 +
padding: 0px;
 +
list-style-type: none;
 +
background: #666666;
 +
}
 +
.ddsmoothmenu ul li{
 +
position: relative;
 +
display: block;
 +
float: left;
 +
}
 +
.ddsmoothmenu ul li a{
 +
line-height: 140%;
 +
overflow: hidden;
 +
text-align: left;
 +
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;
 +
word-spacing: 1px;
 +
font-size: 14px;
 +
font-weight: normal;
 +
display: block; /*background of menu items (default state)*/
 +
padding: 34px 20px 10px 17px;
 +
text-decoration: none;
 +
}
 +
.ddsmoothmenu ul li a span {
 +
font-weight: normal;
 +
color: #888;
 +
font-size: 10px;
 +
clear: both;
 +
display:block;
 +
width: 60px;
 +
}
 +
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/
 +
display: inline-block;
 +
}
 +
 
 +
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{
 +
color: #333;
 +
}
 +
 
 +
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/
 +
color: #888;
 +
}
 +
 
 +
.ddsmoothmenu ul li a:hover{
 +
color: #888;
 +
}
 +
 +
/*1st sub level menu*/
 +
.ddsmoothmenu ul li ul{
 +
position: absolute;
 +
left: 0;
 +
visibility: hidden;
 +
display: none; /*collapse all sub menus to begin with*/
 +
border: 1px #222 solid;
 +
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
}
 +
 
 +
/*Sub level menu list items (undo style from Top level List Items)*/
 +
.ddsmoothmenu ul li ul li{
 +
text-align:left;
 +
float: none;
 +
background: url('img/bg-menu2.png');
 +
}
 +
 
 +
.ddsmoothmenu ul li ul li a{
 +
color: #fff;
 +
font-size: 12px;
 +
}
 +
.ddsmoothmenu ul li ul li  a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/
 +
color: #fff;
 +
}
 +
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{
 +
color: #fff;
 +
}
 +
.ddsmoothmenu ul li ul li a:hover{
 +
color: #ffc;
 +
}
 +
.ddsmoothmenu ul li ul li ul{
 +
top: 0;
 +
}
 +
 
 +
/* Sub level menu links style */
 +
.ddsmoothmenu ul li ul li a{
 +
width: 160px; /*width of sub menus*/
 +
padding: 0px 0px 0px 10px;
 +
height: 38px;
 +
line-height: 320%;
 +
border-left: 1px #555 solid;
 +
overflow: hidden;
 +
}
 +
 
 +
/* Holly Hack for IE \*/
 +
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/
 +
 
 +
.copyright{
 +
width: 930px;
 +
color: #959595;
 +
background: #eee;
 +
clear: both;
 +
line-height: 190%;
 +
text-align: right;
 +
padding: 10px 20px 10px;
 +
margin: 0px auto 0px;
 +
}
 +
.copyright a{
 +
color: #bbb;
 +
text-decoration: none;
 +
}
 +
.copyright a:hover{
 +
color: #ccc;
 +
}
 +
.bar-title-whole{
 +
overflow: visible;
 +
position: relative;
 +
width: 990px;
 +
margin: auto;
 +
z-index: 10;
 +
}
 +
.bar-title{
 +
background: #999 url('http://2011.igem.org/wiki/images/e/e9/Bar-title-bg.jpg') top;
 +
border-left: 1px #8a8a8a solid;
 +
border-right: 1px #8a8a8a solid;
 +
 
 +
width: 918px;
 +
height: 122px;
 +
margin: auto;
 +
overflow: hidden;
 +
padding-left: 35px;
 +
padding-right: 35px;
 +
color: #ddd;
 +
line-height: 150%;
 +
font-size: 12px;
 +
 +
position: relative;
 +
z-index: 10;
 +
}
 +
 
 +
.bar-title h2{
 +
color: #fff;
 +
margin-top: 15px;
 +
margin-bottom: 30px !important;
 +
}
 +
.bar-title h5{
 +
color: #eee;
 +
}
 +
.bar-title-sh-left{
 +
width: 10px;
 +
height: 112px;
 +
background: url('http://2011.igem.org/wiki/images/6/69/Bar-title-sh-left.png') no-repeat;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
left: 0px;
 +
}
 +
.bar-title-sh{
 +
width: 970px;
 +
height: 8px;
 +
background: url('http://2011.igem.org/wiki/images/3/37/Bar-title-sh.png') repeat-x;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
left: 10px;
 +
}
 +
.bar-title-sh-right{
 +
width: 10px;
 +
height: 112px;
 +
background: url('http://2011.igem.org/wiki/images/c/c6/Bar-title-sh-right.png') no-repeat;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
right: 0px;
 +
}
 +
#page{
 +
    width: 970px;
 +
    margin: 0px auto 0px;
 +
    overflow: hidden;
 +
    background: #f9f9f9;
 +
   
 +
}
 +
 
 +
#page .page-wrapper{
 +
margin: 15px 30px 0px 30px;
 +
width: 910px;
 +
overflow: hidden;
 +
float: left;
 +
}
 +
#page .page-wrapper-right{
 +
margin: 30px 40px 0px 25px;
 +
width: 935px;
 +
overflow: hidden;
 +
float: right;
 +
}
 +
#page .title-wrapper h1{
 +
margin-top: 0.5em;
 +
margin-bottom: 0.3em;
 +
}
 +
#page .meta-left-full{
 +
margin: 15px 0px 5px 0px;
 +
overflow: hidden;
 +
}
 +
#page .meta-left-button{
 +
margin: 5px 0px 60px 0px;
 +
overflow: hidden;
 +
}
 +
#page .w600{
 +
width: 600px;
 +
}
 +
 +
</style>
 +
 
 +
 
 +
</head>
 +
<body>
 +
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script>
 +
 
 +
<script>
 +
$(document).ready(
 +
ddsmoothmenu.init({
 +
mainmenuid: "topmenu",
 +
orientation: 'h',
 +
classname: 'ddsmoothmenu',
 +
contentsource: "markup"
 +
}));
 +
 
 +
jQuery(window).load(function() {
 +
setTimeout(function(){
 +
jQuery('#slider').nivoSlider({
 +
pauseTime:4000,
 +
animSpeed:600,
 +
captionOpacity: 0.8,
 +
pauseOnHover:true,
 +
directionNav:true,
 +
directionNavHide:true,
 +
controlNavThumbs:true,
 +
controlNavThumbsFromRel:true,
 +
});
 +
}, 1000),
 +
setTimeout(function(){
 +
/* For slider shortcode */
 +
jQuery('#slider2').nivoSlider({
 +
pauseTime:4000,
 +
animSpeed:600,
 +
captionOpacity: 0.8,
 +
pauseOnHover:true,
 +
directionNavHide:true });
 +
}, 1000);
 +
 +
});
 +
</script>
 +
 
 +
<div class="body-2">
 +
<!-- Header -->
 +
<div id="header-whole">
 +
<div id="header">
 +
<a href="http://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="http://2011.igem.org/wiki/images/9/9c/Igem_basic_logo.png"/></a>
 +
<!-- Logo -->
 +
<div>
 +
<div class="logo">
 +
<a href="http://2011.igem.org/Team:Fatih_Turkey">
 +
<img width="258" src="http://2011.igem.org/wiki/images/b/b6/Fatih_turkey_logo.png">
 +
</a>
 +
</div>
 +
</div>
 +
<!-- Menu -->
 +
<div class="nav-wrapper">
 +
<div class="nav-main">
 +
<div id="topmenu" class="ddsmoothmenu">
 +
<ul id="menu-main" class="menu">
 +
<li>
 +
<a href="http://2011.igem.org/Team:Fatih_Turkey">Home</a>
 +
</li>
 +
<li>
 +
<a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a>
 +
<ul>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Results">Results</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="http://2011.igem.org/Team:Fatih_Turkey/Biobricks">Biobricks</a>
 +
<ul>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Devices">Devices</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Modelling">Modelling</a></li>
 +
</ul>
 +
</li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
 +
<li>
 +
<a href="http://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
 +
<ul>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="http://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a>
 +
<ul>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li>
 +
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li>
 +
</ul>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- End Header -->
 +
<div class="bar-title-whole">
 +
  <div class="bar-title">
 +
      <h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h2>
 +
      <h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5>
 +
    </div>
 +
  <div class="bar-title-sh-left"></div>
 +
  <div class="bar-title-sh"></div>
 +
  <div class="bar-title-sh-right"></div>
 +
        </div>
 +
</div>
 +
<div id="container">
 +
<div id="page">
 +
<div class="page-wrapper">
 +
<!-- post container -->
 +
<div>
 +
<div class="meta-left-full">
 +
<img src="http://igem.org/wiki/images/d/db/M1.jpg" /><br>
 +
1: This part includes a gram positive promoter, an RBS sequence and SacB signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/c/cd/M2.jpg" /><br>
 +
2:This part includes a gram positive promoter, an RBS sequence and LipA signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/7/77/M3.jpg" /><br>
 +
3: This part includes a constitutive promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/5/5d/M4.jpg" /><br>
 +
4:This part includes a IPTG inducible promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/4/49/M5.jpg" /><br>
 +
5:Our “LALF (limulus anti-lipopolysaccharide factor)” protein allows stopping any kind of gram negative bacteria growth by binding their cell wall material, LPS. This image shows that indicated part is considered as confirmed.
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/6/6c/M6.jpg" /><br>
 +
6:Our “reflectin” protein has the ability to reflect the light by changing its wavelength, thus its color. In our project, we planned to use this protein as an indicator on E.coli whether our LALF protein works properly or not. This image shows that indicated part is considered as confirmed.
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/6/6c/M7.jpg" /><br>
 +
8:This part is a well-studied gram positive promoter. We planned to use this gene in the case of our signal peptide sequences do not work. When the protein, which is attached to this gene, is produced, we planned to blow up the bacteria; therefore synthesized protein would be in supernatant. This image shows that indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
<img src="http://igem.org/wiki/images/0/07/M8.jpg" /><br>
 +
500: To perform cloning perfectly, we used pSB1C3 in E.coli. This vector is also the main vector for our parts that works only in E.coli. All of our parts are also inserted into this vector; because it is declared that all parts must be sent to Registry in pSB1C3. This image shows that this part is confirmed.
 +
 
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/c/c0/Ms4.png" /><br>
 +
501: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and SacB signal peptide sequence. In the image, it can be seen that this part is confirmed.
 +
 
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/6/61/M10.jpg" /><br>
 +
502: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and LipA signal peptide sequence. In the image, it can be seen that this part is confirmed.
 +
 
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/4/48/M11.jpg" /><br>
 +
505:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our LALF protein. In the image, it can be seen that this part is confirmed.
 +
 
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/a/aa/M12.jpg" /><br>
 +
506:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our reflectin protein. In the image, it can be seen that this part is confirmed.
 +
 
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/2/25/M13.jpg" /><br>
 +
800: In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectinprotein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has ampicillin resistance for E.coli and chloramphenicol resistance for B.subtilis. In the image, it can be seen that this part is confirmed.
 +
 
 +
<hr>
 +
<img src="http://2011.igem.org/wiki/images/a/a0/Ms3.png" /><br>
 +
900:In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectin protein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has chloramphenicol resistance for both of bacteria kinds. This part also includes an RFP sequence. In the image, it can be seen that this part is confirmed.
 +
<hr>
 +
 
 +
<img src="http://igem.org/wiki/images/a/a5/M16.jpg" /><br>
 +
545: This part is designed; because it is wanted that all parts must be sent to headquarters in pSB1C3 vector. It possesses a promoter that works in gram negative bacteria, Tat signal sequence in order to synthesize the protein outside and LALF protein gene.  In this image, indicated part is considered as confirmed.
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/6/64/Ms2.png" /><br>
 +
815: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 800 that isdesigned for gram positive. In this image, indicated part is considered as confirmed
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/6/6c/Ms1.png" /><br>
 +
915: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/5/54/M18.jpg" /><br>
 +
925:In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, LipA signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/3/30/M19.jpg" /><br>
 +
516: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector. In this image, indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/5/5c/M20.jpg" /><br>
 +
526: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector.In this image, indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/d/d9/M21.jpg" /><br>
 +
536:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. A constitutive promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
 
 +
<img src="http://2011.igem.org/wiki/images/3/3b/M22.jpg" /><br>
 +
546:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. An IPTG inducible promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
 
 +
<img src="http://igem.org/wiki/images/8/85/M23.jpg" /><br>
 +
596:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. We designed an alternative part in the case that our signal sequences do not work. This part includes a promoter that works in only gram negative and reflectin protein gene. All components are inserted into pSB1C3. In this image, indicated part is considered as confirmed.
 +
 
 +
<hr>
 +
 
 +
 
 +
 
 +
</div>
 +
    </div>
 +
      </div>
 +
</div>
 +
</div>
 +
 
 +
<div class="footer-sh-wrapper">
 +
<div class="footer-sh-left"></div>
 +
<div class="footer-sh"></div>
 +
<div class="footer-sh-right"></div>
 +
</div>
 +
<div id="footer">
 +
<style>
 +
#footer img:hover{
 +
opacity:0.6;
 +
}
 +
</style>
 +
<div>
 +
<h3 style="color:#BBB;">Sponsors</h3>
 +
<img height="100" src="http://2011.igem.org/wiki/images/7/72/Tubitak_-_Kopya.png"/>
 +
<a href="http://www.sentegen.com">
 +
<img height="100" src="http://2011.igem.org/wiki/images/2/2d/Sentegennnn.png"/></a>
 +
</div>
 +
</div>
 +
<div class="copyright-open">
 +
</div>
 +
<div class="copyright">
 +
2011 © Fatih Medical School
 +
</div>
 +
</div>
 +
 
 +
 
 +
</body>
 +
</html>

Revision as of 22:29, 21 September 2011

deneme baslik


1: This part includes a gram positive promoter, an RBS sequence and SacB signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.

2:This part includes a gram positive promoter, an RBS sequence and LipA signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.

3: This part includes a constitutive promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.

4:This part includes a IPTG inducible promoter, an RBS sequence and Tat signal peptide sequence to synthesize the protein outside of the cell. This image shows that indicated part is considered as confirmed.

5:Our “LALF (limulus anti-lipopolysaccharide factor)” protein allows stopping any kind of gram negative bacteria growth by binding their cell wall material, LPS. This image shows that indicated part is considered as confirmed.

6:Our “reflectin” protein has the ability to reflect the light by changing its wavelength, thus its color. In our project, we planned to use this protein as an indicator on E.coli whether our LALF protein works properly or not. This image shows that indicated part is considered as confirmed.

8:This part is a well-studied gram positive promoter. We planned to use this gene in the case of our signal peptide sequences do not work. When the protein, which is attached to this gene, is produced, we planned to blow up the bacteria; therefore synthesized protein would be in supernatant. This image shows that indicated part is considered as confirmed.

500: To perform cloning perfectly, we used pSB1C3 in E.coli. This vector is also the main vector for our parts that works only in E.coli. All of our parts are also inserted into this vector; because it is declared that all parts must be sent to Registry in pSB1C3. This image shows that this part is confirmed.

501: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and SacB signal peptide sequence. In the image, it can be seen that this part is confirmed.

502: To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and LipA signal peptide sequence. In the image, it can be seen that this part is confirmed.

505:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our LALF protein. In the image, it can be seen that this part is confirmed.

506:To perform ligation procedure appropriately, we designed an alternative part for all of our material parts. We inserted our promoters and proteins into pSB1C3 in order to have the alternative part that possesses different antibiotic resistance, comparing to original vectors of the parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes the sequence of our reflectin protein. In the image, it can be seen that this part is confirmed.

800: In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectinprotein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has ampicillin resistance for E.coli and chloramphenicol resistance for B.subtilis. In the image, it can be seen that this part is confirmed.

900:In our project, we planned to use gram positive bacteria to synthesize our LALF protein in order to stop gram negative growth. On the other hand, to identify effectively whether our protein works or not, we decided to use reflectin protein and to produce it from both of our bacteria; B.subtilis and E.coli. Thus, we determined to use a shuttle vector that works both bacteria kinds. This vector has chloramphenicol resistance for both of bacteria kinds. This part also includes an RFP sequence. In the image, it can be seen that this part is confirmed.

545: This part is designed; because it is wanted that all parts must be sent to headquarters in pSB1C3 vector. It possesses a promoter that works in gram negative bacteria, Tat signal sequence in order to synthesize the protein outside and LALF protein gene. In this image, indicated part is considered as confirmed.

815: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 800 that isdesigned for gram positive. In this image, indicated part is considered as confirmed

915: In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, SacB signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.

925:In order to stop gram negative bacteria growth, our LALF protein must be synthesized by another type of bacteria, gram positive. Thus, we designed a special gene that works in only gram positive bacteria by inserting a promoter specialized for gram positive. Also, LipA signal sequence that allows us synthesizing the protein outside and LALF protein gene is included.All components are inserted into 900 that is designed for gram positive. In this image, indicated part is considered as confirmed.

516: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector. In this image, indicated part is considered as confirmed.

526: This part is designed; because all parts must be sent to headquarters in pSB1C3 vector.In this image, indicated part is considered as confirmed.

536:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. A constitutive promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.

546:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. This part is designed to work in gram negative E.coli. An IPTG inducible promoter, a Tat signal sequence that allows synthesizing the protein outside and reflectin protein gene is included in pSB1C3. In this image, indicated part is considered as confirmed.

596:In our experiments, we aimed to indicate whether our LALF protein works or not, we used an alternative indicator protein, reflectin. We designed an alternative part in the case that our signal sequences do not work. This part includes a promoter that works in only gram negative and reflectin protein gene. All components are inserted into pSB1C3. In this image, indicated part is considered as confirmed.